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Hypovirus papain-like protease p29 suppresses RNA silencing in the natural fungal host and in a heterologous plant system
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Segers, Gerrit C., van Wezel, Rene, Zhang, Xuemei , Hong, Yiguo and Nuss, Donald L.. (2006) Hypovirus papain-like protease p29 suppresses RNA silencing in the natural fungal host and in a heterologous plant system. Eukaryotic Cell, Vol.5 (No.6). pp. 896-904. ISSN 1535-9778
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Official URL: http://dx.doi.org/10.1128/EC.00373-05
Abstract
Virulence-attenuating hypoviruses of the species Cryphonectria hypovirus 1 (CHV1) encode a papain-like protease, p29, that shares similarities with the potyvirus-encoded suppressor of RNA silencing HC-Pro. We now report that hypovirus CRV1-EP713-encoded p29 can suppress RNA silencing in the natural host, the chestnut blight fungus Cryphonectria parasitica. Hairpin RNA-triggered silencing was suppressed in C parasitica strains expressing p29, and transformation of a transgenic green fluorescent protein (GFP)-silenced strain with p29 resulted in an increased number of transformants with elevated GFP expression levels. The CHV1-EP713 p29 protein was also shown to suppress both virus-induced and agroinfiltration-induced RNA silencing and systemic spread of silencing in GFP-expressing transgenic Nicotiana benthamiana line 16c plants. The demonstration that a mycovirus encodes a suppressor of RNA silencing provides circumstantial evidence that RNA silencing in fungi may serve as an antiviral defense mechanism. The observation that a phylogenetically conserved protein of related plant and fungal viruses functions as a suppressor of RNA silencing in both fungi and plants indicates a level of conservation of the mechanisms underlying RNA silencing in these two groups of organisms.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Science > Life Sciences (2010- ) Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) |
| Journal or Publication Title: | Eukaryotic Cell |
| Publisher: | American Society for Microbiology |
| ISSN: | 1535-9778 |
| Date: | June 2006 |
| Volume: | Vol.5 |
| Number: | No.6 |
| Number of Pages: | 9 |
| Page Range: | pp. 896-904 |
| Identification Number: | 10.1128/EC.00373-05 |
| Status: | Not Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Public Health Service (US) (PHS) |
| Grant number: | GM55981 (PHS) |
| URI: | http://wrap.warwick.ac.uk/id/eprint/33407 |
Data sourced from Thomson Reuters' Web of Knowledge
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