UNSPECIFIED. (2006) Development of a medium for in vitro culture of Galanthus species based on the mineral composition of bulbs. JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, 81 (3). pp. 537-545. ISSN 1462-0316

Abstract

To optimise conditions for micropropagating Galanthus species, a basal medium (G) was developed based on mineral analyses of G. nivalis, G. nivalis 'Flore Pleno' and G. elwesii bulbs. Compared with Murashige and Skoog (MS) medium. the main features of G medium were increased concentrations of Cu ( x 30.4), P ( x 3.6), Ca ( x 1.9), Mg ( x 1.3) and S ( x 1.2) and reduced levels of Mn ( x 0.07), Zn ( x 0.59) and K ( x 0.65). The efficacy of G medium in supporting bulblet initiation on bulb chip explants, bulblet multiplication (on media supplemented with 30 g l(-1) sucrose, 1.0 mg l(-1) 6-benzylaminopurine and 0.1 mg l(-1) naphthalene acetic acid), and bulblet growth (on plant growth regulator-free media with 60 g C sucrose and 5 g C activated charcoal) was compared with MS medium over a range of dilutions (full-, 1/2-, 1/4-, and 1/8-strength). Bulblet initiation was superior on G medium for G. nivalis and G. nivalis 'Flore Pleno', but inferior for G. elwesii. The choice of basal medium did not influence bulblet multiplication, although multiplication was reduced on both media diluted to 1/8-strength. G medium supported bulblet growth and rooting better than MS medium, while dilution of either medium reduced bulblet growth and rooting. Using G medium in place of MS medium during bulblet multiplication greatly reduced hyperhydration with G. elwesii, as did dilution of either of the basal media.

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