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The use of small interfering RNAs to inhibit adipocyte differentiation in human preadipocytes and fetal-femur-derived mesenchymal cells
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Xu, Y., Mirmalek-Sani, S-H., Yang, X., Zhang, J. and Oreffo, R.O.C. (2006) The use of small interfering RNAs to inhibit adipocyte differentiation in human preadipocytes and fetal-femur-derived mesenchymal cells. Experimental Cell Research, Vol.312 (No.10). pp. 1856-1864. doi:10.1016/j.yexcr.2006.02.016
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Official URL: http://dx.doi.org/10.1016/j.yexcr.2006.02.016
Abstract
RNA interference (RNAi) has been used in functional genomics and offers innovative approaches in the development of novel therapeutics. Human mesenchymal stem cells offer a unique cell source for tissue engineering/regeneration strategies. The current study examined the potential of small interfering RNAs (siRNA) against human peroxisome proliferator activated receptor gamma (PPAR gamma) to suppress adipocyte differentiation (adipogenesis) in human preadipocytes and fetal-femur-derived mesenchymal cells. Adipogenesis was investigated using cellular and biochemical analysis. Transient transfection with PPAR gamma-siRNA using a liposomal-based strategy resulted in a significant inhibition of adipogenesis in human preadipocytes and fetal-femur-derived mesenchymal cells, compared to controls (cell, liposomal and negative siRNA). The inhibitory effect of PPAR gamma-siRNA was supported by testing human PPAR gamma mRNA and adipogenic associated genes using reverse transcription polymerase chain reaction (RT-PCR) to adiponectin receptor 1 and 2 as well as examination of fatty acid binding protein 3 (FABP(3)) expression, an adipocyte-specific marker. The current studies indicate that PPAR gamma-siRNA is a useful tool to study adipogenesis in human cells, with potential applications both therapeutic and in the elucidation of mesenchymal cell differentiation in the modulation of cell differentiation in human mesenchymal cells. (c) 2006 Elsevier Inc. All rights reserved.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) Q Science > QH Natural history > QH301 Biology |
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| Divisions: | Faculty of Medicine > Warwick Medical School | ||||
| Journal or Publication Title: | Experimental Cell Research | ||||
| Publisher: | Academic Press | ||||
| ISSN: | 0014-4827 | ||||
| Official Date: | 10 June 2006 | ||||
| Dates: |
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| Volume: | Vol.312 | ||||
| Number: | No.10 | ||||
| Number of Pages: | 9 | ||||
| Page Range: | pp. 1856-1864 | ||||
| DOI: | 10.1016/j.yexcr.2006.02.016 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) |
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