UNSPECIFIED. (2006) Gas phase characterization of the noncovalent quaternary structure of cholera toxin and the cholera toxin B subunit pentamer. BIOPHYSICAL JOURNAL, 90 (9). pp. 3246-3254. ISSN 0006-3495

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Abstract

Cholera toxin (CTx) is an AB(5) cytotonic protein that has medical relevance in cholera and as a novel mucosal adjuvant. Here, we report an analysis of the noncovalent homopentameric complex of CTx B chain ( CTx B-5) using electrospray ionization triple quadrupole mass spectrometry and tandem mass spectrometry and the analysis of the noncovalent hexameric holotoxin usingelectrospray ionization time-of-flight mass spectrometry over a range of pH values that correlate with those encountered by this toxin after cellular uptake. We show that noncovalent interactions within the toxin assemblies were maintained under both acidic and neutral conditions in the gas phase. However, unlike the related Escherichia coli Shiga-like toxin B-5 pentamer (SLTx B), the CTx B-5 pentamer was stable at low pH, indicating that additional interactions must be present within the latter. Structural comparison of the CTx B monomer interface reveals an additional alpha-helix that is absent in the SLTx B monomer. In silico energy calculations support interactions between this helix and the adjacent monomer. These data provide insight into the apparent stabilization of CTx B relative to SLTx B.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology
Journal or Publication Title:	BIOPHYSICAL JOURNAL
Publisher:	BIOPHYSICAL SOCIETY
ISSN:	0006-3495
Date:	May 2006
Volume:	90
Number:	9
Number of Pages:	9
Page Range:	pp. 3246-3254
Identification Number:	10.1529/biophysj.105.076455
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/33709

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