UNSPECIFIED (2006) Diversity of soluble methane monooxygenase-containing methanotrophs isolated from polluted environments. FEMS MICROBIOLOGY LETTERS, 255 (2). pp. 225-232. doi:10.1111/j.1574-6968.2005.00090.x ISSN 0378-1097.

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Abstract

Methanotrophs were enriched and isolated from polluted environments in Canada and Germany. Enrichments in low copper media were designed to specifically encourage growth of soluble methane monooxygenase (sMMO) containing organisms. The 10 isolates were characterized physiologically and genetically with one type I and nine type II methanotrophs being identified. Three key genes: 16S rRNA; pmoA and mmoX, encoding for the particulate and soluble methane monooxygenases respectively, were cloned from the isolates and sequenced. Phylogenetic analysis of these sequences identified strains, which were closely related to Methylococcus capsulatus, Methylocystis sp., Methylosinus sporium and Methylosinus trichosporium. Diversity of sMMO-containing methanotrophs detected in this and previous studies was rather narrow, both genetically and physiologically, suggesting possible constraints on genetic diversity of sMMO due to essential conservation of enzyme function.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	FEMS MICROBIOLOGY LETTERS
Publisher:	BLACKWELL PUBLISHING
ISSN:	0378-1097
Official Date:	February 2006
Dates:	Date Event February 2006 UNSPECIFIED
Volume:	255
Number:	2
Number of Pages:	8
Page Range:	pp. 225-232
DOI:	10.1111/j.1574-6968.2005.00090.x
Publication Status:	Published

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