The mechanism distinguishability problem in biochemical kinetics: The single-enzyme, single-substrate reaction as a case study
UNSPECIFIED. (2006) The mechanism distinguishability problem in biochemical kinetics: The single-enzyme, single-substrate reaction as a case study. COMPTES RENDUS BIOLOGIES, 329 (1). pp. 51-61. ISSN 1631-0691Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.crvi.2005.09.005
A theoretical analysis of the distinguishability problem of two rival models of the single enzyme-single substrate reaction, the Michaelis-Menten and Henri mechanisms, is presented. We also outline a general approach for analysing the structural indisfinguishability between two mechanisms. The approach involves constructing, if possible. a smooth mapping between the two candidate models. Evans et al. [N.D. Evans, M.J. Chappell, MJ. Chapman, K.R. Godfrey, Structural indistinguishability between uncontrolled (autonomous) nonlinear analytic systems, Automatica 40 (2004) 1947-1953] have shown that if, in addition, either of the mechanisms satisfies a particular criterion then such a transformation always exists when the models are indistinguishable from their experimentally observable outputs. The approach is applied to the single enzyme-single substrate reaction mechanism. In principle, mechanisms can be distinguished using this analysis, but we show that our ability to distinguish mechanistic models depends both on the precise measurements made, and on our knowledge of the system prior to performing the kinetics experiments.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Journal or Publication Title:||COMPTES RENDUS BIOLOGIES|
|Publisher:||ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER|
|Official Date:||January 2006|
|Number of Pages:||11|
|Page Range:||pp. 51-61|
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