Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta
UNSPECIFIED. (2005) Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta. JOURNAL OF CELL SCIENCE, 118 (22). pp. 5279-5289. ISSN 0021-9533Full text not available from this repository.
Official URL: http://dx.doi.org/10.1242/jcs.02647
Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower levels of expression can activate signalling without forming puncta. The structures do not localise with markers of the early or late endocytic pathway and time-lapse analysis demonstrates that Dvl-2 puncta move in a random fashion over short distances but do not originate from the plasma membrane. Based on our findings, we propose that Dvl-2 puncta are protein aggregates that are not required for signalling.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Journal or Publication Title:||JOURNAL OF CELL SCIENCE|
|Publisher:||COMPANY OF BIOLOGISTS LTD|
|Date:||15 November 2005|
|Number of Pages:||11|
|Page Range:||pp. 5279-5289|
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