UNSPECIFIED. (2005) Specific in vivo binding of activator of G protein signalling 1 to the G(beta 1) subunit. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 337 (4). pp. 1038-1046. ISSN 0006-291X

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Abstract

Activator of G protein signalling I (AGS1) is a Ras-like protein that affects signalling through heterotrimeric G proteins. Previous in vitro studies suggest that AGS1 can bind to G(alpha)-GDP subunits and promote nucleotide exchange, leading to activation of intracellular signalling pathways. This model is consistent with in vivo evidence demonstrating that AGS1 activates both G(alpha)- and G(beta gamma)-dependent pathways in the absence of ligand. However, it does not easily explain how AGS1 blocks G(beta gamma)-dependent, but not G(alpha)-dependent, signalling following receptor activation. We have used yeast two hybrid analysis and co-immunoprecipitation studies in mammalian cells to demonstrate a direct interaction between AGS1 and the G(beta 1) subunit of heterotrimeric G proteins. The interaction is specific for G(beta 1), and involves the cationic region of AGS1 and the C-terminal region of G(beta 1). Possible implications of this novel interaction for the activity of AGS1 are discussed. (c) 2005 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology
Journal or Publication Title:	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
ISSN:	0006-291X
Date:	2 December 2005
Volume:	337
Number:	4
Number of Pages:	9
Page Range:	pp. 1038-1046
Identification Number:	10.1016/j.bbrc.2005.09.149
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/34368

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