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Specific in vivo binding of activator of G protein signalling 1 to the G(beta 1) subunit
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UNSPECIFIED (2005) Specific in vivo binding of activator of G protein signalling 1 to the G(beta 1) subunit. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 337 (4). pp. 1038-1046. doi:10.1016/j.bbrc.2005.09.149 ISSN 0006-291X.
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Official URL: http://dx.doi.org/10.1016/j.bbrc.2005.09.149
Abstract
Activator of G protein signalling I (AGS1) is a Ras-like protein that affects signalling through heterotrimeric G proteins. Previous in vitro studies suggest that AGS1 can bind to G(alpha)-GDP subunits and promote nucleotide exchange, leading to activation of intracellular signalling pathways. This model is consistent with in vivo evidence demonstrating that AGS1 activates both G(alpha)- and G(beta gamma)-dependent pathways in the absence of ligand. However, it does not easily explain how AGS1 blocks G(beta gamma)-dependent, but not G(alpha)-dependent, signalling following receptor activation. We have used yeast two hybrid analysis and co-immunoprecipitation studies in mammalian cells to demonstrate a direct interaction between AGS1 and the G(beta 1) subunit of heterotrimeric G proteins. The interaction is specific for G(beta 1), and involves the cationic region of AGS1 and the C-terminal region of G(beta 1). Possible implications of this novel interaction for the activity of AGS1 are discussed. (c) 2005 Elsevier Inc. All rights reserved.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
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Journal or Publication Title: | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | ||||
Publisher: | ACADEMIC PRESS INC ELSEVIER SCIENCE | ||||
ISSN: | 0006-291X | ||||
Official Date: | 2 December 2005 | ||||
Dates: |
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Volume: | 337 | ||||
Number: | 4 | ||||
Number of Pages: | 9 | ||||
Page Range: | pp. 1038-1046 | ||||
DOI: | 10.1016/j.bbrc.2005.09.149 | ||||
Publication Status: | Published |
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