UNSPECIFIED. (2005) Regulated expression of putative membrane progestin receptor homologues in human endometrium and gestational tissues. JOURNAL OF ENDOCRINOLOGY, 187 (1). pp. 89-101. ISSN 0022-0795

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Abstract

Rapid non-genomic actions of progesterone are implicated in many aspects of female reproduction. Recently, three human homologues of the fish membrane progestin receptor (mPR) have been identified. We combined bioinformatic analysis with expression profiling to define further the role of these mPRs in human reproductive tissues. Sequence analysis confirmed that the mPRs belong to a larger, highly conserved family of proteins, termed 'progestin and adiponectin receptors' (PAQRs). A comparison of the expression of mPR, transcripts with that of two related PAQR family members, PAQRIII and PAQRIX, in cycling endometrium and pregnancy tissues revealed markedly divergent expression levels and profiles. For instance. endometrial expression of mPR alpha and gamma and PAQRIX was cycle-dependent whereas the onset of parturition was associated with a marked reduction in myometrial mPR alpha and beta transcripts. Interestingly, mPR alpha and PAQRIX were most highly expressed in the placenta, and the tissue expression levels of both genes correlated inversely with that of the nuclear PR. Phylogenetic analysis demonstrated that PAQRIX belongs to the mPR subgroup of proteins. We also validated a polyclonal antibody raised against the carboxy-terminus of human mPRa. Immunohistochemical analysis demonstrated more intense immunoreactivity in placental syncytiotrophoblasts than in endometrial glands or stroma. The data suggest important functional roles for mPR alpha, and possibly PAQRIX, in specific reproductive tissues, particularly those that express low levels of nuclear PR.

Item Type:	Journal Article
Subjects:	R Medicine > RC Internal medicine
Journal or Publication Title:	JOURNAL OF ENDOCRINOLOGY
Publisher:	SOC ENDOCRINOLOGY
ISSN:	0022-0795
Date:	October 2005
Volume:	187
Number:	1
Number of Pages:	13
Page Range:	pp. 89-101
Identification Number:	10.1677/joe.1.06242
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/34439

Data sourced from Thomson Reuters' Web of Knowledge

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