UNSPECIFIED. (2005) Nucleolar localization of potato leafroll virus capsid proteins. JOURNAL OF GENERAL VIROLOGY, 86 (Part 10). pp. 2891-2896. ISSN 0022-1317

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Abstract

Potato leafroll virus (PLRV) encodes two capsid proteins, major protein (CP) and minor protein (P5), an extended version of the CP produced by occasional translational 'readthrough' of the CP gene. Immunogold electron microscopy showed that PLRV CP is located in the cytoplasm and also localized in the nucleus, preferentially targeting the nucleolus. The nucleolar localization of PLRV CP was also confirmed when it was expressed as a fusion with green fluorescent protein (GFP) via an Agrobacterium vector. Mutational analysis identified a particular sequence within PLRV CP involved in nucleolar targeting [the nucleolar localization signal (NoLS)]. Minor protein P5 also contains the same NoLS, and was targeted to the nucleolus when it was expressed as a fusion with GFP from Agrobacterium. However, P5-GFP lost its nucleolar localization in the presence of replicating PLRV.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title:	JOURNAL OF GENERAL VIROLOGY
Publisher:	SOC GENERAL MICROBIOLOGY
ISSN:	0022-1317
Date:	October 2005
Volume:	86
Number:	Part 10
Number of Pages:	6
Page Range:	pp. 2891-2896
Identification Number:	10.1099/vir.0.81101-0
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/34504

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