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Nucleolar localization of potato leafroll virus capsid proteins
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UNSPECIFIED (2005) Nucleolar localization of potato leafroll virus capsid proteins. JOURNAL OF GENERAL VIROLOGY, 86 (Part 10). pp. 2891-2896. doi:10.1099/vir.0.81101-0
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Official URL: http://dx.doi.org/10.1099/vir.0.81101-0
Abstract
Potato leafroll virus (PLRV) encodes two capsid proteins, major protein (CP) and minor protein (P5), an extended version of the CP produced by occasional translational 'readthrough' of the CP gene. Immunogold electron microscopy showed that PLRV CP is located in the cytoplasm and also localized in the nucleus, preferentially targeting the nucleolus. The nucleolar localization of PLRV CP was also confirmed when it was expressed as a fusion with green fluorescent protein (GFP) via an Agrobacterium vector. Mutational analysis identified a particular sequence within PLRV CP involved in nucleolar targeting [the nucleolar localization signal (NoLS)]. Minor protein P5 also contains the same NoLS, and was targeted to the nucleolus when it was expressed as a fusion with GFP from Agrobacterium. However, P5-GFP lost its nucleolar localization in the presence of replicating PLRV.
| Item Type: | Journal Article | ||||
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| Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology |
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| Journal or Publication Title: | JOURNAL OF GENERAL VIROLOGY | ||||
| Publisher: | SOC GENERAL MICROBIOLOGY | ||||
| ISSN: | 0022-1317 | ||||
| Official Date: | October 2005 | ||||
| Dates: |
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| Volume: | 86 | ||||
| Number: | Part 10 | ||||
| Number of Pages: | 6 | ||||
| Page Range: | pp. 2891-2896 | ||||
| DOI: | 10.1099/vir.0.81101-0 | ||||
| Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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