Inflammatory responses to pneumovirus infection in IFN-alpha BR gene-deleted mice
UNSPECIFIED. (2005) Inflammatory responses to pneumovirus infection in IFN-alpha BR gene-deleted mice. JOURNAL OF IMMUNOLOGY, 175 (7). pp. 4735-4744. ISSN 0022-1767Full text not available from this repository.
Pneumonia virus of mice (PVM; family Paramyxoviridae) is a natural pathogen of rodents that reproduces important clinical features of severe respiratory syncytial virus infection in humans. As anticipated, PVM infection induces transcription of IFN antiviral response genes preferentially in wild-type over IFN-alpha beta R gene-deleted (IFN-alpha beta R-/-) mice. However, we demonstrate that PVM infection results in enhanced expression of eotaxin-2 (CCL24), thymus and activation-regulated chemokine (CCL17), and the proinflammatory RNase mouse eosinophil-associated RNase (mEar) 11, and decreased expression of monocyte chemotactic protein-5, IFN-gamma-inducible protein-10, and TLR-3 in lung tissue of IFN-alpha beta R-/- mice when compared with wild type. No differential expression of chemokines MIP-1 alpha or MIP-2 or Th2 cytokines IL-4 or IL-5 was observed. Differential expression of proinflammatory mediators was associated with distinct patterns of lung pathology. The widespread granulocytic infiltration and intra-alveolar edema observed in PVM-infected, wild-type mice are replaced with patchy, dense inflammatory foci localized to the periphery of the larger blood vessels. Bronchoalveolar lavage fluid from IFN-alpha beta R-/- mice yielded 7- to 8-fold fewer leukocytes overall, with increased percentages of eosinophils, monocytes, and CD4(+) T cells, and decreased percentage of CD8(+) T cells. Differential pathology is associated with prolonged survival of the IFN-alpha beta R-/- mice (50% survival at 10.8 +/- 0.6 days vs the wild type at 9.0 +/- 0.3 days; p < 0.02) despite increased virus titers. Overall, our findings serve to identify novel transcripts that are differentially expressed in the presence or absence of IFN-alpha beta R-mediated. signaling, further elucidating interactions between the IFN and antiviral inflammatory responses in vivo.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology > QR180 Immunology|
|Journal or Publication Title:||JOURNAL OF IMMUNOLOGY|
|Publisher:||AMER ASSOC IMMUNOLOGISTS|
|Date:||1 October 2005|
|Number of Pages:||10|
|Page Range:||pp. 4735-4744|
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