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Identification of a novel type of spacer element required for imprinting in fission yeast

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Sayrac, Suha, Vengrova, Sonya, Godfrey, Emma L. and Dalgaard, Jacob Z.. (2011) Identification of a novel type of spacer element required for imprinting in fission yeast. PLoS Genetics, Vol.7 (No.3). e1001328. ISSN 1553-7404

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Official URL: http://dx.doi.org/10.1371/journal.pgen.1001328

Abstract

Asymmetrical segregation of differentiated sister chromatids is thought to be important for cellular differentiation in higher eukaryotes. Similarly, in fission yeast, cellular differentiation involves the asymmetrical segregation of a chromosomal imprint. This imprint has been shown to consist of two ribonucleotides that are incorporated into the DNA during laggingstrand synthesis in response to a replication pause, but the underlying mechanism remains unknown. Here we present key novel discoveries important for unravelling this process. Our data show that cis-acting sequences within the mat1 cassette mediate pausing of replication forks at the proximity of the imprinting site, and the results suggest that this pause dictates specific priming at the position of imprinting in a sequence-independent manner. Also, we identify a novel type of cis-acting spacer region important for the imprinting process that affects where subsequent primers are put down after the replication fork is released from the pause. Thus, our data suggest that the imprint is formed by ligation of a not-fullyprocessed Okazaki fragment to the subsequent fragment. The presented work addresses how differentiated sister chromatids are established during DNA replication through the involvement of replication barriers.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI) Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Yeast, Schizosaccharomyces pombe, Chromosome replication, Cell differentiation
Journal or Publication Title:	PLoS Genetics
Publisher:	Public Library of Science
ISSN:	1553-7404
Date:	10 March 2011
Volume:	Vol.7
Number:	No.3
Page Range:	e1001328
Identification Number:	10.1371/journal.pgen.1001328
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Marie Curie Cancer Care
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URI:	http://wrap.warwick.ac.uk/id/eprint/34580