Sayrac, Suha, Vengrova, Sonya, Godfrey, Emma L. and Dalgaard, Jacob Z. (2011) Identification of a novel type of spacer element required for imprinting in fission yeast. PLoS Genetics, Vol.7 (No.3). e1001328. doi:10.1371/journal.pgen.1001328

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Abstract

Asymmetrical segregation of differentiated sister chromatids is thought to be important for cellular differentiation in higher
eukaryotes. Similarly, in fission yeast, cellular differentiation involves the asymmetrical segregation of a chromosomal
imprint. This imprint has been shown to consist of two ribonucleotides that are incorporated into the DNA during laggingstrand
synthesis in response to a replication pause, but the underlying mechanism remains unknown. Here we present key
novel discoveries important for unravelling this process. Our data show that cis-acting sequences within the mat1 cassette
mediate pausing of replication forks at the proximity of the imprinting site, and the results suggest that this pause dictates
specific priming at the position of imprinting in a sequence-independent manner. Also, we identify a novel type of cis-acting
spacer region important for the imprinting process that affects where subsequent primers are put down after the
replication fork is released from the pause. Thus, our data suggest that the imprint is formed by ligation of a not-fullyprocessed
Okazaki fragment to the subsequent fragment. The presented work addresses how differentiated sister
chromatids are established during DNA replication through the involvement of replication barriers.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Yeast, Schizosaccharomyces pombe, Chromosome replication, Cell differentiation
Journal or Publication Title:	PLoS Genetics
Publisher:	Public Library of Science
ISSN:	1553-7404
Official Date:	10 March 2011
Dates:	Date Event 10 March 2011 Published
Volume:	Vol.7
Number:	No.3
Page Range:	e1001328
DOI:	10.1371/journal.pgen.1001328
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Marie Curie Cancer Care

Data sourced from Thomson Reuters' Web of Knowledge

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