. (2010) Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana. Molecular Systems Biology, Vol.6 (No.397). ISSN 1744-4292

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Abstract

Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little is known about the molecular complexes steering cell division. In a targeted proteomics approach, we mapped the core complex machinery at the heart of the Arabidopsis thaliana cell cycle control. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up- and downstream pathways. Over 100 new candidate cell cycle proteins were predicted and an in-depth biological interpretation demonstrated the hypothesis-generating power of the interaction data. The data set provided a comprehensive view on heterodimeric cyclin-dependent kinase (CDK)–cyclin complexes in plants. For the first time, inhibitory proteins of plant-specific B-type CDKs were discovered and the anaphase-promoting complex was characterized and extended. Important conclusions were that mitotic A- and B-type cyclins form complexes with the plant-specific B-type CDKs and not with CDKA;1, and that D-type cyclins and S-phase-specific A-type cyclins seem to be associated exclusively with CDKA;1. Furthermore, we could show that plants have evolved a combinatorial toolkit consisting of at least 92 different CDK–cyclin complex variants, which strongly underscores the functional diversification among the large family of cyclins and reflects the pivotal role of cell cycle regulation in the developmental plasticity of plants.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Growth (Plants) -- Molecular aspects, Arabidopsis thaliana -- Growth, Arabidopsis thaliana -- Genetics, Cyclin-dependent kinases, Plant cell cycle
Journal or Publication Title:	Molecular Systems Biology
Publisher:	Nature Publishing Group
ISSN:	1744-4292
Date:	10 August 2010
Volume:	Vol.6
Number:	No.397
Identification Number:	10.1038/msb.2010.53
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Agentschap voor Innovatie door Wetenschap en Technologie (Belgium) (IWT), Belgium. Politique scientifique fédérale [Belgian State Science Policy Office] (BELSPO), European Commission (EC), National Science Foundation (U.S.) (NSF), Fonds Wetenschappelijk Onderzoek (FWO)
Grant number:	GBOU 20193 (IWT), AGRON-OMICS LSHG-CT-2006-037704 (EC), IOS 0744566 (NSF)
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URI:	http://wrap.warwick.ac.uk/id/eprint/3486

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