Griffiths, D. E. and Roberton, A. M.. (1966) Energy-linked reactions in mitochondria : studies on the reduction of NAD+ by succinate. Biochimica et Biophysica Acta. Enzymology and Biological Oxidation, Vol.113 (No.1). pp. 13-26. ISSN 0926-6593

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Abstract

1. The ATP-dependent reduction of NAD+ by succinate catalysed by submitochondrial particles prepared from beef, sheep and pig heart mitochondria has been examined 2. The general features of the reaction are similar to those described by other workers, especially the sensitivity of the reaction to inhibitors and uncouplers of respiration and oxidative phosphorylation such as 2,4-dinitrophenol, Amytal, rote-none and oligomycin 3. The stereospecificity of hydrogen transfer in the ATP-dependent reduction of NAD+ has been investigated using [3H]N AD+ as the hydrogen acceptor and methods are described for the preparation and separation of tritiated pyridine nucleotides 4. Stereospecificity studies show that the terminal reductive step in the energy-linked reduction of NAD+ by succinate is catalysed by an enzyme of B-specificity, possibly the NADH dehydrogenase (EC 1.6.99.3) flavoprotein system. These results also exclude malate dehydrogenase (EC 1.1.1.37), an enzyme of A-specificity, as a participant in the reaction.

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Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Science > Chemistry
Journal or Publication Title:	Biochimica et Biophysica Acta. Enzymology and Biological Oxidation
Publisher:	Elsevier BV
ISSN:	0926-6593
Date:	1966
Volume:	Vol.113
Number:	No.1
Page Range:	pp. 13-26
Status:	Peer Reviewed
Description:	Data sourced from: Butterworth, J. (1966). Report of the Vice Chancellor 1965-66. Coventry : University of Warwick.
URI:	http://wrap.warwick.ac.uk/id/eprint/34941

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