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Energy-linked reactions in mitochondria : studies on the mechanism of the energy-linked transhydrogenase reaction
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Griffiths, D. E. and Roberton, A. M. (1966) Energy-linked reactions in mitochondria : studies on the mechanism of the energy-linked transhydrogenase reaction. Biochimica et Biophysica Acta. Enzymology and Biological Oxidation, Vol.118 (No.3). pp. 453-464.
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Official URL: http://www.sciencedirect.com/science/journal/09266...
Abstract
1. The mechanism of both the energy-linked and non-energy-linked trans-hydrogenase reaction catalysed by submitochondrial particles from beef-heart mitochondria has been examined using 3H- and 14C-labelled pyridine nucleotides as substrates.
2. The energy-linked transhydrogenase reaction does not proceed via a net group transfer of either the adenine or nicotinamide moieties or of phosphate from one pyridine nucleotide to another.
3. Studies with 3H-labelled pyridine nucleotides show conclusively that in the energy-linked transhydrogenase reaction hydrogen is transferred directly from the A locus of NADH to the B locus of NADP+, i.e. the energy-linked transhydrogenase is an A:B transhydrogenase involving direct hydrogen transfer.
4. Studies on the non-energy-linked transhydrogenase show conclusively that the stereochemistry of the non-energy-linked reaction is identical to that of the energy-linked transhydrogenase reaction.
5. When the reaction is carried out in tritiated water there is only a low incorporation of tritium into NADPH thus confirming that the reaction is one involving direct hydrogen transfer.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry | ||||
| Divisions: | Faculty of Science > Chemistry | ||||
| Journal or Publication Title: | Biochimica et Biophysica Acta. Enzymology and Biological Oxidation | ||||
| Publisher: | Elsevier BV | ||||
| ISSN: | 0926-6593 | ||||
| Official Date: | 1966 | ||||
| Dates: |
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| Volume: | Vol.118 | ||||
| Number: | No.3 | ||||
| Page Range: | pp. 453-464 | ||||
| Status: | Peer Reviewed | ||||
| Description: | Data sourced from: Butterworth, J. (1966). Report of the Vice Chancellor 1965-66. Coventry : University of Warwick. |
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