Wall, Mark J., Eason, Robert and Dale, Nicholas (2010) Biosensor measurement of purine release from cerebellar cultures and slices. Purinergic Signalling, Vol.6 (No.3). pp. 339-348. doi:10.1007/s11302-010-9185-8 ISSN 1573-9546.

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Abstract

We have previously described an action-potential
and Ca2+-dependent form of adenosine release in the
molecular layer of cerebellar slices. The most likely source
of the adenosine is the parallel fibres, the axons of granule cells. Using microelectrode biosensors, we have therefore investigated whether cultured granule cells (from postnatal day 7–8 rats) can release adenosine. Although no purine release could be detected in response to focal electrical stimulation, purine (adenosine, inosine or hypoxanthine) release occurred in response to an increase in extracellular K+ concentration from 3 to 25 mM coupled with addition of 1 mM glutamate. The mechanism of purine release was transport from the cytoplasm via an ENT transporter.
This process did not require action-potential firing but
was Ca2+dependent. The major purine released was not
adenosine, but was either inosine or hypoxanthine. In
order for inosine/hypoxanthine release to occur, cultures
had to contain both granule cells and glial cells; neither
cellular component was sufficient alone. Using the same
stimulus in cerebellar slices (postnatal day 7–25), it was
possible to release purines. The release however was not
blocked by ENT blockers and there was a shift in the Ca2+
dependence during development. This data from cultures
and slices further illustrates the complexities of purine
release, which is dependent on cellular composition and
developmental stage.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Adenosine, Cerebellum, Axons, Purines
Journal or Publication Title:	Purinergic Signalling
Publisher:	Springer Netherlands
ISSN:	1573-9546
Official Date:	September 2010
Dates:	Date Event September 2010 Published
Volume:	Vol.6
Number:	No.3
Page Range:	pp. 339-348
DOI:	10.1007/s11302-010-9185-8
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Funder:	Medical Research Council (Great Britain) (MRC)

Data sourced from Thomson Reuters' Web of Knowledge

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