Cools, Katherine, Chope, Gemma A., Hammond, John P., Thompson, Andrew J. and Terry, Leon A. (Leon Alexander). (2011) Onion gene expression in response to ethylene and 1-MCP. Plant Physiology, Vol.156 (No.3). pp. 1639-1652. ISSN 1532-2548

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Abstract

Onion is regarded as a non-climacteric vegetable. In onions, however, ethylene can suppress sprouting while the ethylene binding inhibitor, 1-MCP (1- methylcyclopropene) can also suppress sprout growth yet, it is unknown how ethylene and 1-MCP elicit the same response. In this study, onions were treated with 10 μL L-1 ethylene or 1 μL L-1 1-MCP individually or in combination for 24 h at 20°C before or after curing (six weeks) at 20 or 28°C then stored at 1°C. Following curing, a subset of these same onions was stored separately under continuous air or ethylene (10 μL L- 1) at 1°C Onions treated with ethylene and 1-MCP in combination after curing for 24 h had reduced sprout growth as compared with the control 25 weeks after harvest. Sprout growth following storage beyond 25 weeks was only reduced through continuous ethylene treatment. This observation was supported by a higher proportion of down-regulated genes characterised as being involved in photosynthesis measured using a newly developed onion microarray. Physiological and biochemical data suggested that ethylene was being perceived in the presence of 1-MCP since sprout growth was reduced in onions treated with 1-MCP and ethylene applied in combination but not when applied individually. A cluster of probes representing transcripts up-regulated by 1-MCP alone but down-regulated by ethylene alone or in the presence of 1-MCP support this suggestion. Ethylene and 1-MCP both down52 regulated a probe tentatively annotated as an ethylene receptor as well as EIN3, suggesting that both treatments down-regulate the perception and signalling events of ethylene.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- ) Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Alliaceae -- Physiology, Alliaceae -- Postharvest diseases and injuries, Plants -- Effect of ethylene on
Journal or Publication Title:	Plant Physiology
Publisher:	American Society of Plant Biologists
ISSN:	1532-2548
Date:	18 May 2011
Volume:	Vol.156
Number:	No.3
Page Range:	pp. 1639-1652
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA)
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URI:	http://wrap.warwick.ac.uk/id/eprint/35179

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