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Microvesicle-mediated release of soluble LH/hCG receptor (LHCGR) from transfected cells and placenta explants
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Chambers, Anne E., Stanley, Paul F., Randeva, Harpal S. and Banerjee, Subhasis (2011) Microvesicle-mediated release of soluble LH/hCG receptor (LHCGR) from transfected cells and placenta explants. Reproductive Biology and Endocrinology, Vol.9 (No.64). doi:10.1186/1477-7827-9-64 ISSN 1477-7827.
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Official URL: http://dx.doi.org/10.1186/1477-7827-9-64
Abstract
Placental hCG and pitutary LH transduce signals in target tissues through a common receptor (LHCGR). We
demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from
transfected cells and that natural LHCGR is also secreted from human placental explants. LHCGR recombinant
proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster
Ovary cells in suspension culture. Secretion was minimal up to 72h but by 96h 24-37% of the LHCGR had been
released into the culture medium. The secreted proteins were folded and sensitive to glycosidases suggesting
N-linked glycosylation. Secretion was independent of recombinant size and was mediated via structurally defined
membrane vesicles (50-150nm). Similarly cultured human early pregnancy placental explants also released LHCGR
via microvesicles. These studies provide the first experimental evidence of the possible mechanistic basis of the
secretion of LHCGR.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QP Physiology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Luteinizing hormone -- Receptors, Chorionic gonadotropins -- Receptors, Placenta, Transfection | ||||
Journal or Publication Title: | Reproductive Biology and Endocrinology | ||||
Publisher: | BioMed Central Ltd. | ||||
ISSN: | 1477-7827 | ||||
Official Date: | 15 May 2011 | ||||
Dates: |
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Volume: | Vol.9 | ||||
Number: | No.64 | ||||
DOI: | 10.1186/1477-7827-9-64 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 16 December 2015 | ||||
Date of first compliant Open Access: | 16 December 2015 | ||||
Funder: | Advantage West Midlands (AWM) |
Data sourced from Thomson Reuters' Web of Knowledge
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