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Microvesicle-mediated release of soluble LH/hCG receptor (LHCGR) from transfected cells and placenta explants

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Chambers, Anne E., Stanley, Paul F., Randeva, Harpal S. and Banerjee, Subhasis. (2011) Microvesicle-mediated release of soluble LH/hCG receptor (LHCGR) from transfected cells and placenta explants. Reproductive Biology and Endocrinology, Vol.9 (No.64). ISSN 1477-7827

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Official URL: http://dx.doi.org/10.1186/1477-7827-9-64

Abstract

Placental hCG and pitutary LH transduce signals in target tissues through a common receptor (LHCGR). We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. LHCGR recombinant proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster Ovary cells in suspension culture. Secretion was minimal up to 72h but by 96h 24-37% of the LHCGR had been released into the culture medium. The secreted proteins were folded and sensitive to glycosidases suggesting N-linked glycosylation. Secretion was independent of recombinant size and was mediated via structurally defined membrane vesicles (50-150nm). Similarly cultured human early pregnancy placental explants also released LHCGR via microvesicles. These studies provide the first experimental evidence of the possible mechanistic basis of the secretion of LHCGR.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology
Divisions:	Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI) Faculty of Medicine > Warwick Medical School > Metabolic and Vascular Health Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Luteinizing hormone -- Receptors, Chorionic gonadotropins -- Receptors, Placenta, Transfection
Journal or Publication Title:	Reproductive Biology and Endocrinology
Publisher:	BioMed Central Ltd.
ISSN:	1477-7827
Date:	15 May 2011
Volume:	Vol.9
Number:	No.64
Identification Number:	10.1186/1477-7827-9-64
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Advantage West Midlands (AWM)
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URI:	http://wrap.warwick.ac.uk/id/eprint/35505