Bicatalytic conversion of glycerol to value-added products
Sotenko, Maria V. (2010) Bicatalytic conversion of glycerol to value-added products. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b2491712~S15
The objective of this study was to develop a tandem bio- chemical transformation of a
feedstock material to value-added products. The chosen example is microbial fermentation of
glycerol with subsequent esterification of the intermediate 1,3-propanediol with fatty acids.
The use of biphasic aqueous/organic medium for the bi-catalytic system is the key feature of
In the first part of this work, batch and continuous fermentations of glycerol by Clostridium
Butyricum bacteria were optimized to increase productivity of 1,3-propanediol. In the second
part, several catalysts were screened for mono- and biphasic transformation of 1,3-
propanediol. It was discovered that enzymes are the most suitable catalysts for the tandem
reactions of glycerol to 1,3-propanediol derivatives compared to chemical catalysts. Biphasic
enzymatic esterification of 1,3-propanediol was further optimized using Rhizomucor miehei
lipase. Finally, a segmented flow tubular reactor and hollow fiber membrane contactors were
designed and tested as a concept tandem reactor. The hollow fiber reactor with Rhizomucor
miehei lipase immobilized onto polypropylene membrane was found to be the most effective
in the biphasic linoleic acid/aqueous esterification of 1,3-propanediol.
In general, the demonstrated approach and the developed system can be easily utilized in the
biorefinery processes to avoid extraction and purification of the intermediate products, thus
reducing time, energy and emissions.
|Item Type:||Thesis or Dissertation (PhD)|
|Subjects:||Q Science > QR Microbiology|
|Library of Congress Subject Headings (LCSH):||Glycerin, Fermentation|
|Official Date:||October 2010|
|Institution:||University of Warwick|
|Theses Department:||School of Engineering|
|Extent:||iv, 207 leaves : ill., charts|
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