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Substrate-specific clades of active marine methylotrophs associated with a phytoplankton bloom in a temperate coastal environment

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Neufeld, Josh D. , Boden, Rich, Moussard, Hélène, Schäfer, Hendrik and Murrell, J. C. (J. Colin). (2008) Substrate-specific clades of active marine methylotrophs associated with a phytoplankton bloom in a temperate coastal environment. Applied and Environmental Microbiology, Vol.74 (No.23). pp. 7321-7328. ISSN 0099-2240

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Official URL: http://dx.doi.org/10.1128/AEM.01266-08

Abstract

Marine microorganisms that consume one-carbon (C1) compounds are poorly described, despite their impact on global climate via an influence on aquatic and atmospheric chemistry. This study investigated marine bacterial communities involved in the metabolism of C1 compounds. These communities were of relevance to surface seawater and atmospheric chemistry in the context of a bloom that was dominated by phytoplankton known to produce dimethylsulfoniopropionate. In addition to using 16S rRNA gene fingerprinting and clone libraries to characterize samples taken from a bloom transect in July 2006, seawater samples from the phytoplankton bloom were incubated with 13C-labeled methanol, monomethylamine, dimethylamine, methyl bromide, and dimethyl sulfide to identify microbial populations involved in the turnover of C1 compounds, using DNA stable isotope probing. The [13C]DNA samples from a single time point were characterized and compared using denaturing gradient gel electrophoresis (DGGE), fingerprint cluster analysis, and 16S rRNA gene clone library analysis. Bacterial community DGGE fingerprints from 13C-labeled DNA were distinct from those obtained with the DNA of the nonlabeled community DNA and suggested some overlap in substrate utilization between active methylotroph populations growing on different C1 substrates. Active methylotrophs were affiliated with Methylophaga spp. and several clades of undescribed Gammaproteobacteria that utilized methanol, methylamines (both monomethylamine and dimethylamine), and dimethyl sulfide. rRNA gene sequences corresponding to populations assimilating 13C-labeled methyl bromide and other substrates were associated with members of the Alphaproteobacteria (e.g., the family Rhodobacteraceae), the Cytophaga-Flexibacter-Bacteroides group, and unknown taxa. This study expands the known diversity of marine methylotrophs in surface seawater and provides a comprehensive data set for focused cultivation and metagenomic analyses in the future.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Methylotrophic microorganisms, Marine phytoplankton
Journal or Publication Title:	Applied and Environmental Microbiology
Publisher:	American Society for Microbiology
ISSN:	0099-2240
Date:	10 October 2008
Volume:	Vol.74
Number:	No.23
Page Range:	pp. 7321-7328
Identification Number:	10.1128/AEM.01266-08
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Natural Environment Research Council (Great Britain) (NERC), Natural Sciences and Engineering Research Council of Canada (NSERC)
Grant number:	NE/C001 923/1 (NRC), NE/B501404/1 (NERC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/359