Zur Nedden, Stephanie, Hawley, Simon, Pentland, Naomi, Hardie, D. G. (D. Grahame), Doney, Alexander S. and Frenguelli, Bruno G.. (2011) Intracellular ATP influences synaptic plasticity in area CA1 of rat hippocampus via metabolism to adenosine and activity-dependent activation of adenosine A1 receptors. The Journal of Neuroscience, Vol.31 (No.16). pp. 6221-6234. ISSN 1529-2401

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Abstract

The extent to which brain slices reflect the energetic status of the in vivo brain has been a subject of debate. We addressed this issue to investigate the recovery of energetic parameters and adenine nucleotides in rat hippocampal slices and the influence this has on synaptic transmission and plasticity.Weshow that, although adenine nucleotide levels recover appreciably within 10minof incubation, it takes 3 h for a full recovery of the energy charge (to >= 0.93) and that incubation of brain slices at 34°C results in a significantly higher ATP/AMP ratio and a threefold lower activity of AMP-activated protein kinase compared with slices incubated at room temperature. Supplementation of artificial CSF with D-ribose and adenine (Rib/Ade) increased the total adenine nucleotide pool of brain slices, which, when corrected for the influence of the dead cut edges, closely approached in vivo values. Rib/Ade did not affect basal synaptic transmission or paired-pulse facilitation but did inhibit long-term potentiation (LTP) induced by tetanic or weak theta-burst stimulation. This decrease in LTP was reversed by strong theta-burst stimulation or antagonizing the inhibitory adenosine A1 receptor suggesting that the elevated tissue ATP levels had resulted in greater activity-dependent adenosine release during LTP induction. This was confirmed by direct measurement of adenosine release with adenosine biosensors. These observations provide new insight into the recovery of adenine nucleotides after slice preparation, the sources of loss of such compounds in brain slices, the means by which to restore them, and the functional consequences of doing so.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Neurophysiology, Rats -- Physiology, Adenine nucleotides
Journal or Publication Title:	The Journal of Neuroscience
Publisher:	Society for Neuroscience
ISSN:	1529-2401
Date:	20 April 2011
Volume:	Vol.31
Number:	No.16
Page Range:	pp. 6221-6234
Identification Number:	10.1523/JNEUROSCI.4039-10.2011
Status:	Peer Reviewed
Access rights to Published version:	Restricted or Subscription Access
Funder:	Research into Ageing (Charitable trust), European Commission (EC)
Grant number:	LSHM-CT-2004-005272 (EC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/36117

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