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Discovery of cyanophage genomes which contain mitochondrial DNA polymerase

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Chan, Yi-Wah, Mohr, Remus, Millard, Andrew D., Holmes, Antony B., Larkum, A. W. D., Whitworth, Anna L., Mann, Nicholas H., Scanlan, David J. , Hess, Wolfgang R. and Clokie, Martha R. J.. (2011) Discovery of cyanophage genomes which contain mitochondrial DNA polymerase. Molecular Biology and Evolution, Vol.28 (No.8). pp. 2269-2274. ISSN 0737-4038

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Official URL: http://dx.doi.org/10.1093/molbev/msr041

Abstract

DNA polymerase γ is a family A DNA polymerase responsible for the replication of mitochondrial DNA in eukaryotes. The origins of DNA polymerase γ have remained elusive because it is not present in any known bacterium, though it has been hypothesized that mitochondria may have inherited the enzyme by phage-mediated nonorthologous displacement. Here, we present an analysis of two full-length homologues of this gene which were found in the genomes of two bacteriophages which infect the chlorophyll-d containing cyanobacterium Acaryochloris marina. Phylogenetic analyses of these phage DNA polymerase γ proteins show they branch deeply within the DNA polymerase γ clade and therefore share a common origin with their eukaryotic homologues. We also found homologues of these phage polymerases in the environmental Community Cyberinfrastructure for Advanced Microbial Ecology Research and Analysis (CAMERA) database, which fell in the same clade. An analysis of the CAMERA assemblies containing the environmental homologues together with the filter fraction metadata indicated some of these assemblies may be of bacterial origin. We also show that the phage encoded DNA polymerase γ is highly transcribed as the phage genomes are replicated. These findings provide data which may assist in reconstructing the evolution of mitochondria.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH426 Genetics
Divisions:	Faculty of Science > Chemistry Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	DNA polymerases, Bacteriophages -- Genetics, Mitochondria -- Evolution
Journal or Publication Title:	Molecular Biology and Evolution
Publisher:	Oxford University Press
ISSN:	0737-4038
Date:	18 February 2011
Volume:	Vol.28
Number:	No.8
Page Range:	pp. 2269-2274
Identification Number:	10.1093/molbev/msr041
Status:	Peer Reviewed
Access rights to Published version:	Restricted or Subscription Access
Funder:	Natural Environment Research Council (Great Britain) (NERC), Engineering and Physical Sciences Research Council (EPSRC), University of Warwick. Molecular Organisation and Assembly in Cells, Deutscher Akademischer Austauschdienst (DAAD)
Grant number:	NE/E01089X/1 (NERC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/36677