Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells
Dunn, S., Morrison, E. E., Liverpool, T. B., Molina-Paris , Carmen, Cross, R. A., Alonso, M. (Maria) and Peckham, M.. (2008) Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells. Journal of Cell Science, Vol.121 (No.7). pp. 1085-1095. ISSN 0021-9533Full text not available from this repository.
Official URL: http://dx.doi.org/10.1242/jcs.026492
Kinesin-1 is a molecular transporter that trafficks along microtubules. There is some evidence that kinesin-1 targets specific cellular sites, but it is unclear how this spatial regulation is achieved. To investigate this process, we used a combination of in vivo imaging of kinesin heavy-chain Kif5c (an isoform of kinesin-1) fused to GFP, in vitro analyses and mathematical modelling. GFP-Kif5c fluorescent puncta localised to a subset of microtubules in live cells. These puncta moved at speeds of up to 1 μm second–1 and exchanged into cortically labelled clusters at microtubule ends. This behaviour depended on the presence of a functional motor domain, because a rigor-mutant GFP-Kif5c bound to microtubules but did not move along them. Further analysis indicated that the microtubule subset decorated by GFP-Kif5c was highly stable and primarily composed of detyrosinated tubulin. In vitro motility assays showed that the motor domain of Kif5c moved detyrosinated microtubules at significantly lower velocities than tyrosinated (unmodified) microtubules. Mathematical modelling predicted that a small increase in detyrosination would bias kinesin-1 occupancy towards detyrosinated microtubules. These data suggest that kinesin-1 preferentially binds to and trafficks on detyrosinated microtubules in vivo, providing a potential basis for the spatial targeting of kinesin-1-based cargo transport.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology
Q Science > QP Physiology
|Divisions:||Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology
Faculty of Medicine > Warwick Medical School
|Library of Congress Subject Headings (LCSH):||Microtubules, Cytology -- Research, Kinesin|
|Journal or Publication Title:||Journal of Cell Science|
|Publisher:||The Company of Biologists Ltd.|
|Page Range:||pp. 1085-1095|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Wellcome Trust (London, England), Cancer Research UK (CRUK), Marie Curie Memorial Foundation|
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