Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice
des Georges, Amédée, Katsuki, Miho, Drummond, Douglas R., Osei, Michael, Cross, R. A. and Amos, L. A.. (2008) Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice. Nature Structural and Molecular Biology, Vol.15 (No.10). pp. 1102-1108. ISSN 1545-9993Full text not available from this repository.
Official URL: http://dx.doi.org/10.1038/nsmb.1482
In vitro studies of pure tubulin have suggested that tubulin heterodimers in cells assemble into B-lattice microtubules, where the 8-nm dimers in adjacent protofilaments are staggered by 0.9 nm. This arrangement requires the tube to close by forming a seam with an A-lattice, in which the protofilaments are staggered by 4.9 nm. Here we show that Mal3, an EB1 family tip-tracking protein, drives tubulin to assemble in vitro into exclusively 13-protofilament microtubules with a high proportion of A-lattice protofilament contacts. We present a three-dimensional cryo-EM reconstruction of a purely A-lattice microtubule decorated with Mal3, in which Mal3 occupies the groove between protofilaments and associates closely with one tubulin monomer. We propose that Mal3 promotes assembly by binding to freshly formed tubulin polymer and particularly favors any with A-lattice arrangement. These results reopen the question of microtubule structure in cells.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH426 Genetics
Q Science > QK Botany
|Divisions:||Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology
Faculty of Medicine > Warwick Medical School
|Library of Congress Subject Headings (LCSH):||Microtubules, Schizosaccharomyces pombe|
|Journal or Publication Title:||Nature Structural and Molecular Biology|
|Publisher:||Nature Publishing Group|
|Page Range:||pp. 1102-1108|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Medical Research Council (Great Britain) (MRC), Marie Curie Cancer Care, Cancer Research UK (CRUK)|
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