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The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism

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Braun, Marcus, Drummond, Douglas R., Cross, R. A. and McAinsh, Andrew D. (2009) The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism. Nature Cell Biology, Vol.11 (No.6). pp. 724-730. doi:10.1038/ncb1878

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Official URL: http://dx.doi.org/10.1038/ncb1878

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Abstract

The dynamic organization of microtubules into parallel arrays allows interphase cells to set up multi-lane highways for intracellular transport and M-phase cells to build the mitotic and meiotic spindles. Here we show that a minimally reconstituted system composed of Klp2, a kinesin-14 from the fission yeast Schizosaccharomyces pombe, together with microtubules assembled from purified S. pombe tubulin, autonomously assembles bundles of parallel microtubules. Bundles form by an ATP-dependent sorting mechanism that requires the full-length Klp2 motor. By this mechanism, antiparallel-overlapped microtubules slide over one another until they dissociate from the bundles, whereas parallel-overlapped microtubules are selectively trapped by an energy-dissipating force-balance mechanism. Klp2-driven microtubule sorting provides a robust pathway for the organization of microtubules into parallel arrays. In vivo evidence indicates that Klp2 is required for the proper organization of S. pombe interphase microtubules into bipolar arrays of parallel-overlapped microtubules1, 2, 3, 4, suggesting that kinesin-14-dependent microtubule sorting may have wide biological importance.

Item Type: Journal Item
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QP Physiology
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Kinesin, Microtubules, Schizosaccharomyces pombe, Cells -- Motility
Journal or Publication Title: Nature Cell Biology
Publisher: Nature Publishing Group
ISSN: 1465-7392
Official Date: June 2009
Dates:
DateEvent
June 2009Published
Volume: Vol.11
Number: No.6
Page Range: pp. 724-730
DOI: 10.1038/ncb1878
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Marie Curie Cancer Care, Medical Research Council (Great Britain) (MRC)

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