The Library
The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism
Tools
Tools
Tools
Braun, Marcus, Drummond, Douglas R., Cross, R. A. and McAinsh, Andrew D. (2009) The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism. Nature Cell Biology, Vol.11 (No.6). pp. 724-730. ISSN 1465-7392
Full text not available from this repository.
Official URL: http://dx.doi.org/10.1038/ncb1878
Abstract
The dynamic organization of microtubules into parallel arrays allows interphase cells to set up multi-lane highways for intracellular transport and M-phase cells to build the mitotic and meiotic spindles. Here we show that a minimally reconstituted system composed of Klp2, a kinesin-14 from the fission yeast Schizosaccharomyces pombe, together with microtubules assembled from purified S. pombe tubulin, autonomously assembles bundles of parallel microtubules. Bundles form by an ATP-dependent sorting mechanism that requires the full-length Klp2 motor. By this mechanism, antiparallel-overlapped microtubules slide over one another until they dissociate from the bundles, whereas parallel-overlapped microtubules are selectively trapped by an energy-dissipating force-balance mechanism. Klp2-driven microtubule sorting provides a robust pathway for the organization of microtubules into parallel arrays. In vivo evidence indicates that Klp2 is required for the proper organization of S. pombe interphase microtubules into bipolar arrays of parallel-overlapped microtubules1, 2, 3, 4, suggesting that kinesin-14-dependent microtubule sorting may have wide biological importance.
| Item Type: | Journal Item |
|---|---|
| Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology |
| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology Faculty of Medicine > Warwick Medical School |
| Library of Congress Subject Headings (LCSH): | Kinesin, Microtubules, Schizosaccharomyces pombe, Cells -- Motility |
| Journal or Publication Title: | Nature Cell Biology |
| Publisher: | Nature Publishing Group |
| ISSN: | 1465-7392 |
| Date: | June 2009 |
| Volume: | Vol.11 |
| Number: | No.6 |
| Page Range: | pp. 724-730 |
| Identification Number: | 10.1038/ncb1878 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | Marie Curie Cancer Care, Medical Research Council (Great Britain) (MRC) |
| URI: | http://wrap.warwick.ac.uk/id/eprint/36907 |
Actions (login required)
 |
View Item |
