Jaqaman, K., King, E. M., Amaro, A. C., Winter, J. R., Dorn, J. F., Elliott, Hunter L., Mchedlishvili, N., McClelland, S. E., Porter, I. M., Posch, M., Toso, A., Danuser, G., McAinsh, Andrew D., Meraldi, P. and Swedlow, Jason. (2010) Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases. Journal of Cell Biology, Vol.188 (No.5). pp. 665-679. ISSN 0021-9525

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Abstract

During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Microtubules, Cells -- Motility, Kinesin, Cell separation
Journal or Publication Title:	Journal of Cell Biology
Publisher:	Rockefeller University Press
ISSN:	0021-9525
Date:	8 March 2010
Volume:	Vol.188
Number:	No.5
Page Range:	pp. 665-679
Identification Number:	10.1083/jcb.200909005
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Marie Curie Cancer Care, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung [Swiss National Science Foundation], Eidgenössische Technische Hochschule Zürich, National Institutes of Health (U.S.) (NIH)
Grant number:	GM68956 (NIH)
URI:	http://wrap.warwick.ac.uk/id/eprint/36909

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