
The Library
MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis
Tools
Samora, Catarina P., Mogessie, Binyam, Conway, Leslie, Ross, Jennifer L., Straube, Anne and McAinsh, Andrew D. (2011) MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis. Nature Cell Biology, Vol.13 (No.9). pp. 1040-1050. doi:10.1038/ncb2297 ISSN 1465-7392.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1038/ncb2297
Abstract
Correct positioning of the mitotic spindle is critical to establish the correct cell-division plane. Spindle positioning involves capture of astral microtubules and generation of pushing/pulling forces at the cell cortex. Here we show that the tau-related protein MAP4 and the microtubule rescue factor CLASP1 are essential for maintaining spindle position and the correct cell-division axis in human cells. We propose that CLASP1 is required to correctly capture astral microtubules, whereas MAP4 prevents engagement of excess dynein motors, thereby protecting the system from force imbalance. Consistent with this, MAP4 physically interacts with dynein–dynactin in vivo and inhibits dynein-mediated microtubule sliding in vitro. Depletion of MAP4, but not CLASP1, causes spindle misorientation in the vertical plane, demonstrating that force generators are under spatial control. These findings have wide biological importance, because spindle positioning is essential during embryogenesis and stem-cell homeostasis.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics |
||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||
Library of Congress Subject Headings (LCSH): | Mitosis, Microtubules, Spindle (Cell division) | ||||
Journal or Publication Title: | Nature Cell Biology | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 1465-7392 | ||||
Official Date: | September 2011 | ||||
Dates: |
|
||||
Volume: | Vol.13 | ||||
Number: | No.9 | ||||
Page Range: | pp. 1040-1050 | ||||
DOI: | 10.1038/ncb2297 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Marie Curie Cancer Care, National Science Foundation (U.S.) (NSF) | ||||
Grant number: | DGE-0654128 (NSF) |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
![]() |
View Item |