Dynamic rearrangement of nucleoporins during fungal "open" mitosis
Theisen, Ulrike, Straube, Anne and Steinberg, Gero. (2008) Dynamic rearrangement of nucleoporins during fungal "open" mitosis. Molecular Biology of the Cell, Volume 19 (Number 3). pp. 1230-1240. ISSN 1059-1524
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Official URL: http://dx.doi.org/10.1091/mbc.E07-02-0130
Mitosis in animals starts with the disassembly of the nuclear pore complexes and the breakdown of the nuclear envelope. In contrast to many fungi, the corn smut fungus Ustilago maydis also removes the nuclear envelope. Here, we report on the dynamic behavior of the nucleoporins Nup214, Pom152, Nup133, and Nup107 in this "open" fungal mitosis. In prophase, the nuclear pore complexes disassembled and Nup214 and Pom152 dispersed in the cytoplasm and in the endoplasmic reticulum, respectively. Nup107 and Nup133 initially spread throughout the cytoplasm, but in metaphase and early anaphase occurred on the chromosomes. In anaphase, the Nup107-subcomplex redistributed to the edge of the chromosome masses, where the new envelope was reconstituted. Subsequently, Nup214 and Pom152 are recruited to the nuclear pores and protein import starts. Recruitment of nucleoporins and protein import reached a steady state in G2 phase. Formation of the nuclear envelope and assembly of nuclear pores occurred in the absence of microtubules or F-actin, but not if both were disrupted. Thus, the basic principles of nuclear pore complex dynamics seem to be conserved in organisms displaying open mitosis.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QK Botany
|Divisions:||Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology
Faculty of Science > Life Sciences (2010- )
Faculty of Medicine > Warwick Medical School
|Library of Congress Subject Headings (LCSH):||Cell cycle, Mitosis, Cell division, Fungi -- Cytology, Microbial genetics, Cytogenetics, Eukaryotic cells -- Genetics|
|Journal or Publication Title:||Molecular Biology of the Cell|
|Publisher:||American Society for Cell Biology|
|Official Date:||March 2008|
|Page Range:||pp. 1230-1240|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Studienstiftung des Deutschen Volkes|
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