Slavin, Stacy, De Cuendias, Anne, Ladmiral, V. and Haddleton, David M.. (2011) Biotin functionalized poly(sulfonic acid)s for bioconjugation : in situ binding monitoring by QCM-D. Journal of Polymer Science Part A - Polymer Chemistry, Vol.49 (No.5). pp. 1163-1173. ISSN 0887-624X

Full text not available from this repository.

Abstract

We describe the synthesis of biotin end functionalized poly(sulfonic acid)s via living radical polymerization (LRP) for conjugation to Avidin. Quartz crystal microbalance (QCM-D) and competitive binding studies were used to confirm this conjugation. A biotin initiator for copper-mediated LRP was used to provide acrylamide and methacrylate based polymers with the functional end group. This investigation revealed that 2-acrylamido-2-methyl-1-propanesulfonic acid was not a suitable monomer in its acid form but was successfully used in its sodium salt form. A second monomer, 3-sulfopropylmethacrylate as the potassium salt was also studied and both monomers produced polymers with polydispersities <1.3 and 1.4, respectively. Evolution of molecular weight with respect to time indicated that the polymerization of the acrylamide polymer is controlled. Quartz crystal microbalance with dissipation monitoring was used to confirm that the biotinylated polymers were able to bind to Avidin in situ. The gold surface of a quartz crystal was chemically modified resulting in a stable monolayer of Avidin; the biotinylated polymers were passed over the functionalized surface and their grafting ability was examined. A competitive binding evaluation was undertaken with 2-(4-hydroxyphenylazo)benzoic acid (HABA) dye to provide visual verification of conjugation. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Bioconjugates, Polymerization, Water-soluble polymers, Organic compounds -- Synthesis
Journal or Publication Title:	Journal of Polymer Science Part A - Polymer Chemistry
Publisher:	John Wiley & Sons Ltd.
ISSN:	0887-624X
Date:	March 2011
Volume:	Vol.49
Number:	No.5
Page Range:	pp. 1163-1173
Identification Number:	10.1002/pola.24532
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Engineering and Physical Sciences Research Council (EPSRC), Unilever (Firm), Syngenta Foundation for Sustainable Agriculture
URI:	http://wrap.warwick.ac.uk/id/eprint/36952

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item