Shuttleworth, John (1982) Interferon gene expression in virus-induced human B-lymphoblastoid cells. PhD thesis, University of Warwick.

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Abstract

Virus infection of human cells induces the transient expression
of interferon-α and interferon-β. This thesis presents the results
of investigations into the expression of these interferons in the human
B-lymphobastoid cell line, Namalwa, following induction by Sendai virus.
Quantitative and qualitative changes in the synthesis of these
interferon mRNAs and proteins were investigated in order to determine
how interferon gene expression is controlled. Interferon mRNA was assayed
both by translation in Xenonus oocytes and by hybridization with cloned
interferon cDNA. Interferon was measured both by bioassay and by immunoradiometric
assay using a monoclonal antibody to interferon-α. In
addition the effects of various treatment which perturb the normal control
of interferon production have been assessed.
Sendai virus infection of Namalvra cells resulted in the coordinate
induction and regulation of both interferon-α and interferon-β synthesis.
Although significant amounts of interferon-β were present, the cells
did not produce any functional interferon-β protein. It was concluded
that the interferon-ß mRNA in these cells was inactive.
Production of interferon was shown to be increased by incubating
cells at reduced temperatures following induction. It was concluded that
the normal inactivation and degradation of interferon mRNA which occurs
during the shut-off of interferon production was inhibited at the lower
temperature. This resulted in increased interferon production over a
prolonged period.
Treatment of cells with butyrate or 5'-bromodeoxyuridine before
induction caused a dose-dependant increase in the rate of interferon mRNA
and interferon synthesis. These treatments appear to coordinately affect
the control of both interferon-α and interferon-β gene expression,
since no differences could be detected in the characteristics of interferon
or interferon mRNA produced by treated and untreated cells. The effect
of these treatments was relatively specific, since polyacrylamide gel
electrophoresis of proteins from butyrate- and 5'-bromodeoxyuridine-treated
cells failed to detect any changes which were comprable to or
could account for the effect on interferon synthesis.

Item Type:	Thesis or Dissertation (PhD)
Subjects:	Q Science > QR Microbiology > QR180 Immunology
Library of Congress Subject Headings (LCSH):	Interferon, Lymphoblastoid cell lines, Sendai virus
Official Date:	November 1982
Dates:	Date Event November 1982 Submitted
Institution:	University of Warwick
Theses Department:	Department of Biological Sciences
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Morser, Michael John ; Burke, David C.
Sponsors:	Science Research Council (Great Britain) (SRC)
Extent:	x, 123, [42] p.
Language:	eng
URI:	http://wrap.warwick.ac.uk/id/eprint/3702

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