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Distinct albino3-dependent and -independent pathways for thylakoid membrane protein insertion

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Woolhead, Cheryl A., Thompson, Simon J., Moore, Misty, Tissier, Christophe, Mant, Alexandra, Rodger, Alison, Henry, Ralph and Robinson, Colin. (2001) Distinct albino3-dependent and -independent pathways for thylakoid membrane protein insertion. Journal of Biological Chemistry, Vol.276 (No.44). pp. 40841-40846. ISSN 00219258

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Official URL: http://dx.doi.org/10.1074/jbc.M106523200

Abstract

The homologous proteins Oxa1, YidC and Alb3 mediate the insertion of membrane proteins in mitochondria, bacteria and chloroplast thylakoids, respectively. Depletion of YidC in Escherichia coli affects the integration of every membrane protein studied, and Alb3 has previously been shown to be required for the insertion of a signal recognition particle (SRP)- dependent protein, Lhcb1, in thylakoids. In this study we have analyzed the 'global' role of Alb3 in the insertion of thylakoid membrane proteins. We show that insertion of two chlorophyll-binding proteins, Lhcb4.1 and Lhcb5, is almost totally blocked by preincubation of thylakoids with anti-Alb3 antibodies, indicating a requirement for Alb3 in the insertion pathway. Insertion of the related PsbS protein, on the other hand, is unaffected by Alb3 antibodies, and insertion of a group of SRP-independent, signal peptide-bearing proteins, PsbX, PsbW and PsbY is likewise completely unaffected. Proteinase K is furthermore able to completely degrade Alb3 but this treatment does not affect the insertion of these proteins. Among the thylakoid proteins studied here, Alb3 requirement correlates strictly with a requirement for stromal factors and nucleoside triphosphates. However, the majority of proteins tested do not require Alb3 or any other known form of translocation apparatus.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Membrane proteins, Thylakoids -- Physiology
Journal or Publication Title:	Journal of Biological Chemistry
Publisher:	American Society for Biochemistry and Molecular Biology
ISSN:	00219258
Date:	2 November 2001
Volume:	Vol.276
Number:	No.44
Page Range:	pp. 40841-40846
Identification Number:	10.1074/jbc.M106523200
Status:	Peer Reviewed
Access rights to Published version:	Restricted or Subscription Access
Funder:	Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), National Science Foundation (U.S.) (NSF)
Grant number:	GR/M91105 (EPSRC), C07900 (BBSRC), C12908 (BBSRC), MCB-9807826 (NSF)
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URI:	http://wrap.warwick.ac.uk/id/eprint/37086