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Distinct albino3-dependent and -independent pathways for thylakoid membrane protein insertion
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Woolhead, Cheryl A., Thompson, Simon J., Moore, Misty, Tissier, Christophe, Mant, Alexandra, Rodger, Alison, Henry, Ralph and Robinson, Colin (2001) Distinct albino3-dependent and -independent pathways for thylakoid membrane protein insertion. Journal of Biological Chemistry, Vol.276 (No.44). pp. 40841-40846. doi:10.1074/jbc.M106523200 ISSN 00219258.
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WRAP_Rodger_0582931-ch-240811-2001._68._jbc_276_40841.pdf - Accepted Version - Requires a PDF viewer. Download (909Kb) |
Official URL: http://dx.doi.org/10.1074/jbc.M106523200
Abstract
The homologous proteins Oxa1, YidC and Alb3 mediate the insertion of membrane proteins
in mitochondria, bacteria and chloroplast thylakoids, respectively. Depletion of YidC in
Escherichia coli affects the integration of every membrane protein studied, and Alb3 has
previously been shown to be required for the insertion of a signal recognition particle (SRP)-
dependent protein, Lhcb1, in thylakoids. In this study we have analyzed the 'global' role of
Alb3 in the insertion of thylakoid membrane proteins. We show that insertion of two
chlorophyll-binding proteins, Lhcb4.1 and Lhcb5, is almost totally blocked by preincubation
of thylakoids with anti-Alb3 antibodies, indicating a requirement for Alb3 in the insertion
pathway. Insertion of the related PsbS protein, on the other hand, is unaffected by Alb3
antibodies, and insertion of a group of SRP-independent, signal peptide-bearing proteins,
PsbX, PsbW and PsbY is likewise completely unaffected. Proteinase K is furthermore able to
completely degrade Alb3 but this treatment does not affect the insertion of these proteins.
Among the thylakoid proteins studied here, Alb3 requirement correlates strictly with a
requirement for stromal factors and nucleoside triphosphates. However, the majority of
proteins tested do not require Alb3 or any other known form of translocation apparatus.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QK Botany | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science, Engineering and Medicine > Science > Chemistry |
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Library of Congress Subject Headings (LCSH): | Membrane proteins, Thylakoids -- Physiology | ||||
Journal or Publication Title: | Journal of Biological Chemistry | ||||
Publisher: | American Society for Biochemistry and Molecular Biology | ||||
ISSN: | 00219258 | ||||
Official Date: | 2 November 2001 | ||||
Dates: |
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Volume: | Vol.276 | ||||
Number: | No.44 | ||||
Page Range: | pp. 40841-40846 | ||||
DOI: | 10.1074/jbc.M106523200 | ||||
Status: | Peer Reviewed | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 17 December 2015 | ||||
Date of first compliant Open Access: | 17 December 2015 | ||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), National Science Foundation (U.S.) (NSF) | ||||
Grant number: | GR/M91105 (EPSRC), C07900 (BBSRC), C12908 (BBSRC), MCB-9807826 (NSF) |
Data sourced from Thomson Reuters' Web of Knowledge
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