ANP stimulates hepatocyte Ca2+ efflux via plasma membrane recruitment of PKGIα
Stratton, Rebecca C., Squires, Paul E. and Green, Anne K.. (2008) ANP stimulates hepatocyte Ca2+ efflux via plasma membrane recruitment of PKGIα. Biochemical and Biophysical Research Communications, Vol.368 (No.4). pp. 965-970. ISSN 0006-291XFull text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.bbrc.2008.02.030
In rat hepatocytes, atrial natriuretic peptide (ANP) elevates cGMP through activation of particulate guanylyl cyclase and attenuates Ca2+ signals by stimulating net plasma membrane Ca2+ efflux. We show here that ANP-stimulated hepatocyte Ca2+ efflux is mediated by protein kinase G (PKG) isotype I. Furthermore, we show that ANP recruits endogenous PKGIα, but not PKGIβ, to the plasma membrane. These effects are mimicked by 8-bromo-cGMP, but not by the soluble guanylyl cyclase activators, sodium nitroprusside and YC-1. We propose that ANP, through localized cGMP elevation, promotes plasma membrane recruitment of PKGIα, which, in turn, stimulates Ca2+ efflux.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology
Q Science > QP Physiology
|Divisions:||Faculty of Science > Life Sciences (2010- )
Faculty of Medicine > Warwick Medical School
|Library of Congress Subject Headings (LCSH):||Atrial natriuretic peptides, Liver cells, Cell membranes, Protein kinases|
|Journal or Publication Title:||Biochemical and Biophysical Research Communications|
|Page Range:||pp. 965-970|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||University of Warwick, Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)|
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