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Calibration and standardisation of synchrotron radiation circular dichroism and conventional circular dichroism spectrophotometers
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Miles, Andrew J., Wien, Frank, Lees, Jonathan G., Rodger, Alison and Wallace, Bonnie Ann (2003) Calibration and standardisation of synchrotron radiation circular dichroism and conventional circular dichroism spectrophotometers. Spectroscopy, Vol.17 (No.4). pp. 653-661. ISSN 1875-922X .
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Official URL: https://iospress.metapress.com/content/m0vudyp6fby...
Abstract
Synchrotron radiation circular dichroism (SRCD) is an emerging technique in
structural biology with particular value in protein secondary structure analyses since it
permits the collection of data down to much lower wavelengths than conventional
circular dichroism (cCD) instruments. Reference database spectra collected on different
SRCD instruments in the future as well as current reference datasets derived from cCD
spectra must be compatible. Therefore there is a need for standardization of calibration
methods to ensure quality control. In this study, magnitude and optical rotation
measurements on four cCD and three SRCD instruments were compared at 192.5, 219,
290 and 490 nm. At high wavelengths, all gave comparable results, however, at the lower
wavelengths, some variations were observable. The consequences of these differences on
the spectrum, and the calculated secondary secondary structure, of a representative
protein (myoglobin) are demonstrated. A normalisation method is proposed for
standardising spectra obtained on any CD instrument, conventional or SR-based, with
respect to existing and future databases.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
| Library of Congress Subject Headings (LCSH): | Synchrotron radiation, Circular dichroism, Spectrophotometer -- Calibration | ||||
| Journal or Publication Title: | Spectroscopy | ||||
| Publisher: | I O S Press | ||||
| ISSN: | 1875-922X | ||||
| Official Date: | 2003 | ||||
| Dates: |
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| Volume: | Vol.17 | ||||
| Number: | No.4 | ||||
| Page Range: | pp. 653-661 | ||||
| Status: | Peer Reviewed | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), GlaxoSmithKline, Medical Research Council (Great Britain) (MRC) | ||||
| Grant number: | B13586 (BBSRC), B14225 (BBSRC) |
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