Alix, Karine, Joets, Johann, Ryder, Carol D., Moore, Jonathan D., Barker, Guy C., Bailey, John P., King , Graham J. and Heslop-Harrison, J. (John) (2008) The CACTA transposon Bot1 played a major role in Brassica genome divergence and gene proliferation. Plant Journal, Vol.56 (No.6). pp. 1030-1044. doi:10.1111/j.1365-313X.2008.03660.x ISSN 0960-7412.

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Abstract

We isolated and characterized a Brassica C genome-specific CACTA element, which was designated Bot1 (Brassica oleracea transposon 1). After analysing phylogenetic relationships, copy numbers and sequence similarity of Bot1 and Bot1 analogues in B. oleracea (C genome) versus Brassica rapa (A genome), we concluded that Bot1 has encountered several rounds of amplification in the oleracea genome only, and has played a major role in the recent rapa and oleracea genome divergence. We performed in silico analyses of the genomic organization and internal structure of Bot1, and established which segment of Bot1 is C-genome specific. Our work reports a fully characterized Brassica repetitive sequence that can distinguish the Brassica A and C chromosomes in the allotetraploid Brassica napus, by fluorescent in situ hybridization. We demonstrated that Bot1 carries a host S locus-associated SLL3 gene copy. We speculate that Bot1 was involved in the proliferation of SLL3 around the Brassica genome. The present study reinforces the assumption that transposons are a major driver of genome and gene evolution in higher plants.

Item Type:	Journal Article
Subjects:	S Agriculture > SB Plant culture Q Science > QR Microbiology
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Brassica oleracea, Brassica rapa, Genomics, Transposons
Journal or Publication Title:	Plant Journal
Publisher:	Blackwell
ISSN:	0960-7412
Official Date:	22 August 2008
Dates:	Date Event 22 August 2008 ["eprint_fieldopt_dates_date_type_available" not defined]
Volume:	Vol.56
Number:	No.6
Page Range:	pp. 1030-1044
DOI:	10.1111/j.1365-313X.2008.03660.x
Status:	Peer Reviewed
Access rights to Published version:	Open Access (Creative Commons)
Funder:	Organisation for Economic Co-operation and Development (OECD), National Science Foundation (U.S.) (NSF)

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