Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

The promoter from SlREO, a highly-expressed, root-specific Solanum lycopersicum gene, directs expression to cortex of mature roots

Tools
- Tools
+ Tools

Jones, Matthew O., Manning, Kenneth, Andrews, John, Wright, Carole, Taylor, Ian B. and Thompson, Andrew J. (2008) The promoter from SlREO, a highly-expressed, root-specific Solanum lycopersicum gene, directs expression to cortex of mature roots. Functional Plant Biology, Vol.35 (No.12). pp. 1224-1233. doi:10.1071/FP08139 ISSN 1445-4408.

[img] Text
WRAP_Manning_Root_promoter_paper_Aug_30_MJ.pdf - Accepted Version

Download (764Kb)
Official URL: http://dx.doi.org/10.1071/FP08139

Request Changes to record.

Abstract

Root-specific promoters are valuable tools for targeting transgene expression, but many of those already described have limitations to their general applicability. We present the expression characteristics of SlREO, a novel gene isolated from tomato (Solanum lycopersicum L.). This gene was highly expressed in roots but had a very low level of expression in aerial plant organs. A 2.4-kb region representing the SlREO promoter sequence was cloned upstream of the uidA GUS reporter gene and shown to direct expression in the root cortex. In mature, glasshouse-grown plants this strict root specificity was maintained. Furthermore, promoter activity was unaffected by dehydration or wounding stress but was somewhat suppressed by exposure to NaCl, salicylic acid and jasmonic acid. The predicted protein sequence of SlREO contains a domain found in enzymes of the 2-oxoglutarate and Fe(II)-dependent dioxygenase superfamily. The novel SlREO promoter has properties ideal for applications requiring strong and specific gene expression in the bulk of tomato root tissue growing in soil, and is also likely to be useful in other Solanaceous crops

Item Type: Journal Article
Subjects: S Agriculture > SB Plant culture
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH): Roots (Botany) -- Research, Genetic engineering
Journal or Publication Title: Functional Plant Biology
Publisher: CSIRO
ISSN: 1445-4408
Official Date: 16 December 2008
Dates:
DateEvent
16 December 2008Published
Volume: Vol.35
Number: No.12
Page Range: pp. 1224-1233
DOI: 10.1071/FP08139
Status: Peer Reviewed
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 1 January 2016
Date of first compliant Open Access: 1 January 2016
Funder: Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA)
Grant number: HP0218

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us