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Mobile FT mRNA contributes to the systemic florigen signalling in floral induction

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Li, Chunyang, Gu, Mei, Shi, Nongnong, Zhang, Hang, Yang, Xin, Osman, Toba, Liu, Yule, Wang, Huizhong, Vatish, Manu, Jackson, Stephen D. and Hong, Yiguo . (2011) Mobile FT mRNA contributes to the systemic florigen signalling in floral induction. Scientific Reports, Vol.1 (No.73). pp. 1-7. ISSN 2045-2322

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Official URL: http://dx.doi.org/10.1038/srep00073

Abstract

In inducing photoperiodic conditions, plants produce a signal dubbed "florigen" in leaves. Florigen moves through the phloem to the shoot apical meristem (SAM) where it induces flowering. In Arabidopsis, the FLOWERING LOCUS T (FT) protein acts as a component of this phloem-mobile signal. However whether the transportable FT mRNA also contributes to systemic florigen signaling remains to be elucidated. Using non-conventional approaches that exploit virus-induced RNA silencing and meristem exclusion of virus infection, we demonstrated that the Arabidopsis FT mRNA, independent of the FT protein, can move into the SAM. Viral ectopic expression of a non-translatable FT mRNA promoted earlier flowering in the short-day (SD) Nicotiana tabacum Maryland Mammoth tobacco in SD. These data suggest a possible role for FT mRNA in systemic floral signalling, and also demonstrate that cis-transportation of cellular mRNA into SAM and meristem exclusion of pathogenic RNAs are two mechanistically distinct processes.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI) Faculty of Science > Life Sciences (2010- ) Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Arabidopsis -- Genetics, Plants, Flowering of -- Genetics, Messenger RNA
Journal or Publication Title:	Scientific Reports
Publisher:	Nature Publishing Group
ISSN:	2045-2322
Date:	24 August 2011
Volume:	Vol.1
Number:	No.73
Page Range:	pp. 1-7
Identification Number:	10.1038/srep00073
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	University of Warwick, Guo jia zi ran ke xue ji jin wei yuan hui (China) [National Natural Science Foundation of China] (NSFC), Hangzhou da xue, Chengdu Institute of Biological Products
Grant number:	30770185 (NSFC), 30870180 (NSFC), 31070298 (NSFC), 20090232T05 (Hangzhou)
References:	1. Zeevaart, J. A. D. Physiology of Flower Formation. Annu. Rev. Plant Physiol. 27, 321-348 (1976). 2. Corbesier, L. & Coupland, G. The quest for florigen: a review of recent progress. J. Exp. Bot. 57, 3395-3403 (2006). 3. Tatada, S. & Goto, K. TERMINAL FLOWER2, an Arabidopsis homolog of HETEROCHROMATIN PROTEIN1, counteracts the activation of FLOWERING LOCUS T by CONSTANS in the vascular tissues of leaves to regulate flowering time. Plant Cell 15, 2856-2865 (2003). 4. Abe, M. et al. FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex. Science 309, 1052-1056 (2005). 5. An, H. et al. CONSTANS acts in the phloem to regulate a systemic signal that induces photoperiodic flowering of Arabidopsis. Development 131, 3615-3526 (2004). 6. Wigge, P. A. et al. Integration of spatial and temporal information during floral induction in Arabidopsis. Science 309, 1056-1059 (2005). 7. Corbesier, L. et al. FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis. Science 316, 1030-1033 (2007). 8. Jaeger, K. E. & Wigge, P. A. FT protein acts as a long-range signal in Arabidopsis. Curr. Biol. 17, 1050-1054 (2007). 9. Mathieu, J., Warthmann, N., Küttner, F. & Schmid, M. Export of FT protein from phloem companion cells is sufficient for floral induction in Arabidopsis. Curr. Biol. 17, 1055-1060 (2007). 10. Notaguchi, M. et al. Long-distance, graft-transmissible action of Arabidopsis FLOWERING LOCUS T protein to promote flowering. Plant Cell Physiol. 49, 1645-1658 (2008). 11. Tamaki, S., Matsuo, S., Wong, H. L., Yokoi, S. & Shimamoto, K. Hd3a protein is a mobile flowering signal in rice. Science 316, 1033-1036 (2007). 12. Lin, M. K. et al. FLOWERING LOCUS T protein may act as the long-distance florigenic signal in the Cucurbits. Plant Cell 19, 1488-1506 (2007). 13. Lifschitz, E. et al. The tomato FT ortholog triggers systemic signals that regulate growth and flowering and substitute for diverse environmental stimuli. Proc. Natl. Acad. Sci. USA 103, 6398-6403 (2006). 14. Li, C. et al. A cis element within Flowering Locus T mRNA determines its mobility and facilitates trafficking of heterologous viral RNA. J. Virol. 83, 3540-3548 (2009). 15. Kragler, F. RNA in the phloem: A crisis or a return on investment? Plant Sci 178, 99-104 (2010). 16. Yant, L., Mathieu, J. & Schmid, M. Just say no: floral repressors help Arabidopsis bide the time. Curr. Opin. Plant Biol. 12, 580-586 (2009). 17. Kehr, J. & Buhtz, A. Long distance transport and movement of RNA through the phloem. J. Exp. Bot. 59, 85-92 (2008). 18. Deeken, R. et al. Identification of Arabidopsis thaliana phloem RNAs provides a search criterion for phloem-based transcripts hidden in complex datasets of microarray experiments. Plant J. 55, 746-759 (2008). 19. Hannapel, D. J. A model system of development regulated by the long-distance transport of mRNA. J. Integr. Plant Biol. 52, 40-52 (2010). 20. Haywood, V., Yu, T. S., Huang, N. C. & Lucas, W. J. Phloem long-distance trafficking of GIBBERELLIC ACID-INSENSITIVE RNA regulates leaf development. Plant J. 42, 49-68 (2005). 21. Kim, M., Canio, W., Kessler, S. & Sinha, N. Developmental changes due to long-distance movement of a homeobox fusion transcript in tomato. Science 293, 287-289 (2001). 22. Xoconostle-Cázares, B. et al. Plant paralog to viral movement protein that potentiates transport of mRNA into the phloem. Science 283, 94-98 (1999). 23. Foster, T. M. et al. A Surveillance System Regulates Selective Entry of RNA into the Shoot Apex. Plant Cell 14, 1497–1508 (2002). 24. Martín-Hernández, A. M. & Baulcombe, D. C. Tobacco rattle virus 16-kilodalton protein encodes a suppressor of RNA silencing that allows transient viral entry in meristem. J. Virol. 82, 4064-4071 (2008). 25. Mochizuki, T. & Ohki, S. T. Shoot meristem tissue of tobacco inoculated with Cucumber mosaic virus is infected with the virus and subsequently recovers from infection by RNA silencing. J. Gen. Plant Pathol. 70, 363-366 (2004). 26. Qu, F. et al. RDR6 has a broad-spectrum but temperature-dependent antiviral defense role in Nicotiana benthamiana. J. Virol. 79, 15209-15217 (2005). 27. Schwach, F., Vaistij, F. E., Jones, L. & Baulcombe, D. C. An RNA-dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal. Plant Physiol. 138:1842-1852 (2005). 28. Matthews, R. E. F. Plant Virology, Ed3. Academic Press, San Diego (1990). 29. Lucas, W. J. Plant viral movement proteins: agents for cell-to-cell trafficking of viral genomes. Virology 344, 169-184 (2006). 30. Lucas, W. J. et al. Selective trafficking of KNOTTED1 homeodomain protein and its mRNA through plasmodesmata. Science 270, 1980-1983 (1995). 31. Ruiz-Medrano, R., Xoconostle-Cázares, B., Lucas, W. J. Phloem long-distance transport of CmNACP mRNA: implications for supracellular regulation in plants. Development 126, 4405-4419 (1999). 32. Irish, V. F. & Sussex, I. M. A fate map of the Arabidopsis embryonic shoot apical meristem. Development 115, 745-753 (1992). 33. Kim, J. K., Rim, Y., Wang, J. & Jackson, D. A novel cell-to-cell trafficking assay indicates that KNOX homeodomain is necessary and sufficient for intercellular protein and mRNA trafficking. Genes Dev. 19, 788-793 (2005). 34. Hannapel, D. J. A model system of development regulated by the long-distance transport of mRNA. J. Integr. Plant Biol. 52, 40-52 (2010). 35. Ferrandon, D., Koch, I., Westhof, E. & Nusslein-Volhard, C. RNA-RNA interaction is required for the formation of specific bicoid mRNA 3’ UTR-STAUFEN ribonucleoprotein particles. EMBO J. 16, 1751-1758 (1997). 36. Elvira, G., Massie, B. & DesGroseillers, L. The zinc-finger protein ZFR is critical for Staufen 2 isoform specific nucleocytoplasmic shuttling in neurons. J. Neurochem. 96, 105-117 (2006). 37. Wang, Y. & Ding, B. Viroids: Small probes for exploring the vast universe of RNA trafficking in plants. J. Integr. Plant Biol. 52, 28-39 (2010). 38. Huang, N. C. & Yu, T. S. The sequences of Arabidopsis GA-INSENSITIVE RNA constitute the motifs that are necessary and sufficient for RNA long-distance trafficking. Plant J. 59, 921-929 (2009). 39. Ham, B. K., Brandom, J. L., Xoconostle-Cazares, B., Ringgold, V., Lough, T. L. & Lucas, W. J. A polypyrimidine tract binding protein, pumpkin RBP50, forms the basis of a phloem-mobile ribonucleoprotein complex. Plant Cell 21, 197–215 (2009). 40. Li, P., Ham, B. & Lucas, W. J. CmRBP50 protein phosphorylation is essential for assembly of a stable phloem-mobile high-affinity ribonucleoprotein complex. J. Biol. Chem. 286, 23142-23149 (2011). 41. Giavalisco, P., Kapitza, K., Kolasa, A., Buhtz, A. & Kehr, J. Towards the proteome of Brassica napus phleom sap. Proteomics 6, 896-909 (2006).
URI:	http://wrap.warwick.ac.uk/id/eprint/38113