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Short communication : preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk
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Smith, Edward M., Monaghan, E. M. (Emma M.), Huntley, Selene J. and Green, Laura E.. (2011) Short communication : preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk. Journal of Dairy Science, Vol.94 (No.10). pp. 4850-4855. ISSN 00220302
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Official URL: http://dx.doi.org/10.3168/jds.2010-4076
Abstract
The objective of this study was to investigate the recovery of bacteria from ewe milk after freezing for 4 or 8 weeks with and without the addition of glycerol as a cryopreservant. A total of 50 udder-half milk samples with a known range of bacterial species were selected, stored and analyzed in 5 treatment-groups: time zero, frozen for 4 weeks with, and without, glycerol; and frozen for 8 weeks with, and without, glycerol. There was a lower recovery of all bacterial species studied after freezing. Samples containing fewer than 100 cfu/mL came from ewes with a lower somatic cell count and were more likely to be bacteriologically negative after freezing than those above this threshold. The addition of glycerol increased recovery of Gram-negative bacteria after freezing, although this requires further study to draw strong conclusions. The effects on Gram-positive species were inconsistent. We conclude that whilst the addition of glycerol had a small beneficial impact on the sensitivity of detection of bacteria from frozen sheep milk, sensitivity was highest in cultures from fresh milk.
| Item Type: | Journal Article | ||||
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| Alternative Title: | Preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk | ||||
| Subjects: | Q Science > QR Microbiology S Agriculture > SF Animal culture |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Sheep milk -- Analysis, Glycerin, Cryobiology, Mastitis | ||||
| Journal or Publication Title: | Journal of Dairy Science | ||||
| Publisher: | Elsevier | ||||
| ISSN: | 00220302 | ||||
| Official Date: | October 2011 | ||||
| Dates: |
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| Volume: | Vol.94 | ||||
| Number: | No.10 | ||||
| Page Range: | pp. 4850-4855 | ||||
| Identification Number: | 10.3168/jds.2010-4076 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Wellcome Trust (London, England) | ||||
| References: | Arsenault, J., P. Dubreuil, R. Higgins, and D. Belanger. 2008. Risk factors and impacts of clinical and subclinical mastitis in commercial meat-producing sheep flocks in Quebec, Canada. Prev. Vet. Med. 87:373-393. Fortin, M., S. Messier, J. Pare, and R. Higgins. 2003. Identification of catalase-negative, non-beta-hemolytic, gram-positive cocci isolated from milk samples. J. Clin. Microbiol. 41:106-109. Analytical specificity and sensitivity of a real-time polymerase chain reaction assay for identification of bovine mastitis pathogens. J. Dairy Sci. 92:952-959. |
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| URI: | http://wrap.warwick.ac.uk/id/eprint/38305 |
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