Smith, Edward M., Monaghan, E. M. (Emma M.), Huntley, S. J. (Selene J.) and Green, Laura E.. (2011) Short communication : preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk. Journal of Dairy Science, Vol.94 (No.10). pp. 4850-4855. ISSN 00220302

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Abstract

The objective of this study was to investigate the recovery of bacteria from ewe milk after freezing for 4 or 8 weeks with and without the addition of glycerol as a cryopreservant. A total of 50 udder-half milk samples with a known range of bacterial species were selected, stored and analyzed in 5 treatment-groups: time zero, frozen for 4 weeks with, and without, glycerol; and frozen for 8 weeks with, and without, glycerol. There was a lower recovery of all bacterial species studied after freezing. Samples containing fewer than 100 cfu/mL came from ewes with a lower somatic cell count and were more likely to be bacteriologically negative after freezing than those above this threshold. The addition of glycerol increased recovery of Gram-negative bacteria after freezing, although this requires further study to draw strong conclusions. The effects on Gram-positive species were inconsistent. We conclude that whilst the addition of glycerol had a small beneficial impact on the sensitivity of detection of bacteria from frozen sheep milk, sensitivity was highest in cultures from fresh milk.

Item Type:	Journal Article
Alternative Title:	Preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk
Subjects:	Q Science > QR Microbiology S Agriculture > SF Animal culture
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Sheep milk -- Analysis, Glycerin, Cryobiology, Mastitis
Journal or Publication Title:	Journal of Dairy Science
Publisher:	Elsevier
ISSN:	00220302
Date:	October 2011
Volume:	Vol.94
Number:	No.10
Page Range:	pp. 4850-4855
Identification Number:	10.3168/jds.2010-4076
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Wellcome Trust (London, England)
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URI:	http://wrap.warwick.ac.uk/id/eprint/38305

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