Short communication : preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk
Smith, Edward M., Monaghan, E. M. (Emma M.), Huntley, Selene J. and Green, Laura E.. (2011) Short communication : preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk. Journal of Dairy Science, Vol.94 (No.10). pp. 4850-4855. ISSN 00220302
WRAP_Green_0380313-lf-260911-scjds_140411.pdf - Accepted Version
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WRAP_Green_fig1_final_size_210211.pdf - Accepted Version
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WRAP_Green_fig2_final_size_210211.pdf - Accepted Version
Official URL: http://dx.doi.org/10.3168/jds.2010-4076
The objective of this study was to investigate the recovery of bacteria from ewe milk after freezing for 4 or 8 weeks with and without the addition of glycerol as a cryopreservant. A total of 50 udder-half milk samples with a known range of bacterial species were selected, stored and analyzed in 5 treatment-groups: time zero, frozen for 4 weeks with, and without, glycerol; and frozen for 8 weeks with, and without, glycerol. There was a lower recovery of all bacterial species studied after freezing. Samples containing fewer than 100 cfu/mL came from ewes with a lower somatic cell count and were more likely to be bacteriologically negative after freezing than those above this threshold. The addition of glycerol increased recovery of Gram-negative bacteria after freezing, although this requires further study to draw strong conclusions. The effects on Gram-positive species were inconsistent. We conclude that whilst the addition of glycerol had a small beneficial impact on the sensitivity of detection of bacteria from frozen sheep milk, sensitivity was highest in cultures from fresh milk.
|Item Type:||Journal Article|
|Alternative Title:||Preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk|
|Subjects:||Q Science > QR Microbiology
S Agriculture > SF Animal culture
|Divisions:||Faculty of Science > Life Sciences (2010- )|
|Library of Congress Subject Headings (LCSH):||Sheep milk -- Analysis, Glycerin, Cryobiology, Mastitis|
|Journal or Publication Title:||Journal of Dairy Science|
|Official Date:||October 2011|
|Page Range:||pp. 4850-4855|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Wellcome Trust (London, England)|
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Analytical specificity and sensitivity of a real-time polymerase chain reaction assay for identification of bovine mastitis pathogens. J. Dairy Sci. 92:952-959.
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