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Short communication : preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk

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Smith, Edward M., Monaghan, E. M. (Emma M.), Huntley, Selene J. and Green, Laura E.. (2011) Short communication : preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk. Journal of Dairy Science, Vol.94 (No.10). pp. 4850-4855. ISSN 00220302

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Abstract

The objective of this study was to investigate the recovery of bacteria from ewe milk after freezing for 4 or 8 weeks with and without the addition of glycerol as a cryopreservant. A total of 50 udder-half milk samples with a known range of bacterial species were selected, stored and analyzed in 5 treatment-groups: time zero, frozen for 4 weeks with, and without, glycerol; and frozen for 8 weeks with, and without, glycerol. There was a lower recovery of all bacterial species studied after freezing. Samples containing fewer than 100 cfu/mL came from ewes with a lower somatic cell count and were more likely to be bacteriologically negative after freezing than those above this threshold. The addition of glycerol increased recovery of Gram-negative bacteria after freezing, although this requires further study to draw strong conclusions. The effects on Gram-positive species were inconsistent. We conclude that whilst the addition of glycerol had a small beneficial impact on the sensitivity of detection of bacteria from frozen sheep milk, sensitivity was highest in cultures from fresh milk.

Item Type: Journal Article
Alternative Title: Preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk
Subjects: Q Science > QR Microbiology
S Agriculture > SF Animal culture
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Sheep milk -- Analysis, Glycerin, Cryobiology, Mastitis
Journal or Publication Title: Journal of Dairy Science
Publisher: Elsevier
ISSN: 00220302
Official Date: October 2011
Volume: Vol.94
Number: No.10
Page Range: pp. 4850-4855
Identification Number: 10.3168/jds.2010-4076
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Wellcome Trust (London, England)
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URI: http://wrap.warwick.ac.uk/id/eprint/38305

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