Smith, Edward M., Monaghan, E. M. (Emma M.), Huntley, Selene J. and Green, Laura E.. (2011) Short communication : preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk. Journal of Dairy Science, Vol.94 (No.10). pp. 4850-4855. ISSN 00220302

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Abstract

The objective of this study was to investigate the recovery of bacteria from ewe milk after freezing for 4 or 8 weeks with and without the addition of glycerol as a cryopreservant. A total of 50 udder-half milk samples with a known range of bacterial species were selected, stored and analyzed in 5 treatment-groups: time zero, frozen for 4 weeks with, and without, glycerol; and frozen for 8 weeks with, and without, glycerol. There was a lower recovery of all bacterial species studied after freezing. Samples containing fewer than 100 cfu/mL came from ewes with a lower somatic cell count and were more likely to be bacteriologically negative after freezing than those above this threshold. The addition of glycerol increased recovery of Gram-negative bacteria after freezing, although this requires further study to draw strong conclusions. The effects on Gram-positive species were inconsistent. We conclude that whilst the addition of glycerol had a small beneficial impact on the sensitivity of detection of bacteria from frozen sheep milk, sensitivity was highest in cultures from fresh milk.

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Item Type:	Journal Article
Alternative Title:	Preliminary investigation into the effect of freezing and a cryopreservant on the recovery of mastitis pathogens from ewe milk
Subjects:	Q Science > QR Microbiology S Agriculture > SF Animal culture
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Sheep milk -- Analysis, Glycerin, Cryobiology, Mastitis
Journal or Publication Title:	Journal of Dairy Science
Publisher:	Elsevier
ISSN:	00220302
Date:	October 2011
Volume:	Vol.94
Number:	No.10
Page Range:	pp. 4850-4855
Identification Number:	10.3168/jds.2010-4076
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Wellcome Trust (London, England)
References:	Arsenault, J., P. Dubreuil, R. Higgins, and D. Belanger. 2008. Risk factors and impacts of clinical and subclinical mastitis in commercial meat-producing sheep flocks in Quebec, Canada. Prev. Vet. Med. 87:373-393. Boonyayatra, S., L. K. Fox, T. E. Besser, A. Sawant, and J. M. Gay. 2010. Effects of storage methods on the recovery of Mycoplasma species from milk samples. Vet. Microbiol. 144 210-213. Bradley, A. J., M. J. Green, and J. N. Huxley. 2002. Making better use of milk samples: Monitoring and investigating herd mastitis. Cattle Practice 10:105-112. Clements, A. C., D. J. Taylor, and J. L. Fitzpatrick. 2003. Evaluation of diagnostic procedures for subclinical mastitis in meat-producing sheep. J. Dairy Res. 70:139-148. de Lamballerie, X., C. Zandotti, C. Vignoli, C. Bollet, and P. de Micco. 1992. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Res. Microbiol. 143:785-790. Dinsmore, R. P., P. B. English, R. N. Gonzalez, and P. M. Sears. 1992. Use of augmented cultural techniques in the diagnosis of the bacterial cause of clinical bovine mastitis. J. Dairy Sci. 75:2706-2712. Fortin, M., S. Messier, J. Pare, and R. Higgins. 2003. Identification of catalase-negative, non-beta-hemolytic, gram-positive cocci isolated from milk samples. J. Clin. Microbiol. 41:106-109. Fthenakis, G. C. and J. E. Jones. 1990. The effect of experimentally induced subclinical mastitis on milk yield of ewes and on the growth of lambs. Br. Vet. J. 146:43-49. Gillespie, B. E. and S. P. Oliver. 2005. Simultaneous detection of mastitis pathogens, Staphylococcus aureus, Streptococcus uberis, and Streptococcus agalactiae by multiplex real-time polymerase chain reaction. J. Dairy Sci. 88:3510-3518. Gonzalez-Rodriguez, M. C., C. Gonzalo, F. San Primitivo, and P. Carmenes. 1995. Relationship between somatic cell count and intramammary infection of the half udder in dairy ewes. J. Dairy Sci. 78:2753-2759. Gonzalo, C., A. Ariznabarreta, J. A. Carriedo, and F. San Primitivo. 2002. Mammary pathogens and their relationship to somatic cell count and milk yield losses in dairy ewes. J. Dairy Sci. 85:1460-1467. Guenther, S., P. Schierack, M. Grobbel, A. Lubke-Becker, L. H. Wieler, and C. Ewers. 2008. Real-time PCR assay for the detection of species of the genus Mannheimia. J. Microbiol. Methods 75:75-80. Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41:95-98. Hariharan, H., W. Donachie, C. Macaldowie, and G. Keefe. 2004. Bacteriology and somatic cell counts in milk samples from ewes on a Scottish farm. Can. J. Vet. Res. 68:188-192. Hogan, J. S., R. N. Gonzalez, R. J. Harmon, S. C. Nickerson, S. P. Oliver, J. W. Pankey, and K. L. Smith. 1999. Laboratory handbook on bovine mastitis. National Mastitis Council, Madison, WI. International Dairy Federation. 1995. Enumeration of somatic cells. FIL-IDF Standard no. 148A. IDF, Brussels, Belgium. Koskinen, M. T., J. Holopainen, S. Pyorala, P. Bredbacka, A. Pitkala, H. W. Barkema, R. Bexiga, J. Roberson, L. Solverod, R. Piccinini, D. Kelton, H. Lehmusto, S. Niskala, and L. Salmikivi. 2009. Analytical specificity and sensitivity of a real-time polymerase chain reaction assay for identification of bovine mastitis pathogens. J. Dairy Sci. 92:952-959. Moore, J. E., Y. Maeda, J. Xu, B. C. Millar, P. H. Herold, V. M. J. Browne-Lauwers, C. E. Goldsmith, A. Loughrey, P. J. Rooney, J. S. Elborn, and M. Matsuda. 2008. Employment of 16S rDNA gene sequencing techniques for improved identification of difficult-to-identify bacterial veterinary pathogens. World J. Microbiol. Biotechnol. 24:1227-1232. Nickerson, S. C. and R. L. Boddie. 1994. Effect of naturally occurring coagulase-negative staphylococcal infections on experimental challenge with major mastitis pathogens. J. Dairy Sci. 77:2526-2536. Polychroniadou, A. and A. Vafopoulou. 1985. Variations of major mineral constituents of ewe milk during lactation. J. Dairy Sci. 68:147-150. Riffon, R., K. Sayasith, H. Khalil, P. Dubreuil, M. Drolet, and J. Lagace. 2001. Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR. J. Clin. Microbiol. 39:2584-2589. Rodriguez Rodriguez, E. M., M. Sanz Alaejos, and C. Diaz Romero. 2001. Mineral concentrations in cow's milk from the Canary Island. J. Food Compos. Anal. 14:419-430. Schukken, Y. H., F. J. Grommers, J. A. Smit, D. Vandegeer, and A. Brand. 1989. Effect of freezing on bacteriologic culturing of mastitis milk samples. J. Dairy Sci. 72:1900-1906. Schukken, Y. H., K. E. Leslie, D. A. Barnum, B. A. Mallard, J. H. Lumsden, P. C. Dick, G. H. Vessie, and M. E. Kehrli. 1999. Experimental Staphylococcus aureus intramammary challenge in late lactation dairy cows: quarter and cow effects determining the probability of infection. J. Dairy Sci. 82:2393-2401. Sol, J., O. C. Sampimon, E. Hartman, and H. W. Barkema. 2002. Effect of preculture freezing and incubation on bacteriological isolation from subclinical mastitis samples. Vet. Microbiol. 85:241-249. Stackebrandt, E. and B. M. Goebel. 1994. Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bact. 44:846-849. Villanueva, M. R., J. W. Tyler, and M. C. Thurmond. 1991. Recovery of Streptococcus agalactiae and Staphylococcus aureus from fresh and frozen bovine milk. J. A. V. M. A. 198:1398-1400. Watkins, G. H., A. R. Burriel, and J. E. Jones. 1991. A field investigation of subclinical mastitis in sheep in southern England. Br. Vet. J. 147:413-420.
URI:	http://wrap.warwick.ac.uk/id/eprint/38305

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