Drummond, Sheona P., Hildyard, John, Firczuk, Helena, Reamtong, Onrapak, Li, Ning, Kannambath, Shichina, Claydon, Amy J., Beynon, R. J. (Robert J.), Eyers, Claire E. and McCarthy, John E. G. (2011) Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes. Nucleic Acids Research, Vol.39 (No.17). pp. 7764-7774. doi:10.1371/journal.pone.0023630

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Abstract

Dhh1 and Pat1 in yeast are mRNA decapping activators/
translational repressors thought to play key
roles in the transition of mRNAs from translation to
degradation. However, little is known about the
physical and functional relationships between these
proteins and the translation machinery. We describe
a previously unknown type of diauxic shiftdependent
modulation of the intracellular locations
of Dhh1 and Pat1. Like the formation of P bodies,
this phenomenon changes the spatial relationship
between components involved in translation and
mRNA degradation. We report significant spatial
separation of Dhh1 and Pat1 from ribosomes in
exponentially growing cells. Moreover, biochemical
analyses reveal that these proteins are excluded
from polysomal complexes in exponentially growing
cells, indicating that they may not be associated
with active states of the translation machinery. In
contrast, under diauxic growth shift conditions,
Dhh1 and Pat1 are found to co-localize with polysomal
complexes. This work suggests that Dhh1
and Pat1 functions are modulated by a relocalization
mechanism that involves eIF4A. Pulldown
experiments reveal that the intracellular
binding partners of Dhh1 and Pat1 change as cells
undergo the diauxic growth shift. This reveals a
new dimension to the relationship between translation
activity and interactions between mRNA, the
translation machinery and decapping activator
proteins.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Gene expression, Genetic translation, Messenger RNA, Yeast -- Physiology
Journal or Publication Title:	Nucleic Acids Research
Publisher:	Oxford University Press
ISSN:	1362-4962
Official Date:	28 June 2011
Dates:	Date Event 28 June 2011 Published
Volume:	Vol.39
Number:	No.17
Page Range:	pp. 7764-7774
DOI:	10.1371/journal.pone.0023630
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Royal Society (Great Britain)
Grant number:	BB/E015778 (BBSRC), BBF019963/1 (BBSRC)

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