Influence of an m-type thioredoxin in maize on potyviral infection
Shi, Yan, Qin, Yanhong, Cao, Yanyong, Sun, Hu, Zhou, Tao, Hong, Yiguo and Fan, Zaifeng. (2011) Influence of an m-type thioredoxin in maize on potyviral infection. European Journal of Plant Pathology, Vol.131 (No.2). pp. 317-326. ISSN 0929-1873Full text not available from this repository.
Official URL: http://dx.doi.org/10.1007/s10658-011-9810-6
Expression of many host genes can be altered during virus infection. In a previous study of sugarcane mosaic virus (SCMV) infection in maize (Zea mays), we observed that expression of ZmTrm2, a gene encoding thioredoxin m, was up-regulated at about 10 days post-inoculation (dpi). In this present study we determined that ZmTrm2 silencing in maize by virus-induced gene silencing significantly enhanced systemic SCMV infection. In contrast transient over-expression of ZmTrm2 in maize protoplasts inhibited accumulation of SCMV viral RNA. Furthermore, we found that in inoculated Nicotiana tabacum leaves transient expression of ZmTrm2 inhibited accumulation of the RNA of tobacco vein-banding mosaic virus (TVBMV), a potyvirus infecting dicotyledonous plants. Interestingly in ZmTrm2 transiently expressed N. tabacum leaves, we detected by semi-quantitative RT-PCR a reduced level of the mRNA of class I beta-1, 3-glucanase (GluI), a protein known to have a role in cell wall callose deposition and viral movement. Our data indicate that the maize ZmTrm2 plays an inhibitory role during infection of plants by SCMV and TVBMV.
|Item Type:||Journal Article|
|Subjects:||Q Science > QK Botany
Q Science > QP Physiology
S Agriculture > SB Plant culture
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)|
|Library of Congress Subject Headings (LCSH):||Thioredoxin, Corn, Potyvirus diseases|
|Journal or Publication Title:||European Journal of Plant Pathology|
|Page Range:||pp. 317-326|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Guo jia zi ran ke xue ji jin wei yuan hui (China) [National Natural Science Foundation (China)] (NNSF), China. Nong ye bu [China. Ministry of Agriculture] (CMA)|
|Grant number:||30771404 (NNSF), 2008ZX08003-001 (CMA), 2009ZX08003-011B (CMA)|
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