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Differentiating N-terminal aspartic and isoaspartic acid residues in peptides
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Sargaeva, Nadezda P., Lin, Cheng and O’Connor, Peter B. (2011) Differentiating N-terminal aspartic and isoaspartic acid residues in peptides. Analytical Chemistry, Vol.83 (No.17). pp. 6675-6682. doi:10.1021/ac201223d ISSN 0003-2700.
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Official URL: http://dx.doi.org/10.1021/ac201223d
Abstract
Formation of isoaspartic acid (isoAsp) is a common modification of aspartic acid (Asp) or asparagine (Asn) residue in proteins. Differentiation of isoAsp and Asp residues is a challenging task owing to their similar properties and identical molecular mass. It was recently shown that they can be differentiated using ion-electron or ion-ion interaction fragmentation methods (ExD) because these methods provide diagnostic fragments c + 57 and z(center dot) - 57 specific to the isoAsp residue. To date, however, the presence of such fragments has not been explored on peptides with an N-terminal isoAsp residue. To address this question, several N-terminal isoAsp-containing peptides were analyzed using ExD methods alone or combined with chromatography. A diagnostic fragment [M + 2H - 74](+center dot) was observed for the doubly charged precursor ions with N-terminal isoAsp residues. For some peptides, identification of the N-terminal isoAsp residue was challenging because of the low diagnostic ion peak intensity and the presence of interfering peaks. Supplemental activation was used to improve diagnostic ion detection. Further, N-terminal acetylation was offered as a means to overcome the interference problem by shifting the diagnostic fragment peak to [M + 2H - 116](+center dot).
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
| Library of Congress Subject Headings (LCSH): | Tandem mass spectrometry, High performance liquid chromatography, Succinimides, Proteins -- Deamination, Peptides, Aspartic acid, Isoaspartic acid | ||||
| Journal or Publication Title: | Analytical Chemistry | ||||
| Publisher: | American Chemical Society | ||||
| ISSN: | 0003-2700 | ||||
| Official Date: | 1 September 2011 | ||||
| Dates: |
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| Volume: | Vol.83 | ||||
| Number: | No.17 | ||||
| Number of Pages: | 8 | ||||
| Page Range: | pp. 6675-6682 | ||||
| DOI: | 10.1021/ac201223d | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Engineering and Physical Sciences Research Council (EPSRC), National Center for Research Resources (U.S.) (NCRR), National Institutes of Health (U.S.) (NIH), National Institute of General Medical Sciences (U.S.) (NIGMS) | ||||
| Grant number: | EP/F034210/1 (EPSRC), P41 RR10888 (NIH/NCRR), R01GM078293 (NIH/NIGMS), S10 RR025082 (NIH/NCRR) |
Data sourced from Thomson Reuters' Web of Knowledge
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