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Prussian Blue acts as a mediator in a reagentless cytokinin biosensor
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Kowalska, Marta, Tian, Faming, Šmehilová, Mária, Galuszka, Petr, Frébort, Ivo, Napier, R. (Richard) and Dale, Nicholas (2011) Prussian Blue acts as a mediator in a reagentless cytokinin biosensor. Analytica Chimica Acta, Vol.701 (No.2). pp. 218-223. doi:10.1016/j.aca.2011.06.018
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Official URL: http://dx.doi.org/10.1016/j.aca.2011.06.018
Abstract
An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzes an irreversible cleavage of N(6)-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare purified AtCKX2 as the basis of the cytokinin biosensor. Prussian Blue (PrB) was electrodeposited on Pt microelectrodes prior to deposition of the enzyme in a sol-gel matrix. The biosensor gave amperometric responses to several cytokinins. These responses depended on the presence of both the enzyme and the Prussian Blue. Thus Prussian Blue must act as an electron mediator between the FAD centre in CKX2 and the Pt surface.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QK Botany | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Biosensors -- Design, Cytokinins, Prussian blue, Bioelectrochemistry | ||||
| Journal or Publication Title: | Analytica Chimica Acta | ||||
| Publisher: | Elsevier Science BV | ||||
| ISSN: | 0003-2670 | ||||
| Official Date: | 9 September 2011 | ||||
| Dates: |
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| Volume: | Vol.701 | ||||
| Number: | No.2 | ||||
| Page Range: | pp. 218-223 | ||||
| DOI: | 10.1016/j.aca.2011.06.018 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Funder: | Czech Republic. Ministerstvo školství, mládeže a tělovýchovy [Ministry of Education, Youth, and Sport], European Regional Development Fund (ERDF), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
| Grant number: | MSM6198959216 (MSM), CZ.1.05./2.1.00/01.0007 (ERDF), BB/F014651/1 (BBSRC) |
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