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Prussian Blue acts as a mediator in a reagentless cytokinin biosensor

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Kowalska, Marta, Tian, Faming, Šmehilová, Mária, Galuszka, Petr, Frébort, Ivo, Napier, Richard M. and Dale, Nicholas. (2011) Prussian Blue acts as a mediator in a reagentless cytokinin biosensor. Analytica Chimica Acta, Vol.701 (No.2). pp. 218-223. ISSN 0003-2670

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Official URL: http://dx.doi.org/10.1016/j.aca.2011.06.018

Abstract

An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzes an irreversible cleavage of N(6)-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare purified AtCKX2 as the basis of the cytokinin biosensor. Prussian Blue (PrB) was electrodeposited on Pt microelectrodes prior to deposition of the enzyme in a sol-gel matrix. The biosensor gave amperometric responses to several cytokinins. These responses depended on the presence of both the enzyme and the Prussian Blue. Thus Prussian Blue must act as an electron mediator between the FAD centre in CKX2 and the Pt surface.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Biosensors -- Design, Cytokinins, Prussian blue, Bioelectrochemistry
Journal or Publication Title:	Analytica Chimica Acta
Publisher:	Elsevier Science BV
ISSN:	0003-2670
Date:	9 September 2011
Volume:	Vol.701
Number:	No.2
Page Range:	pp. 218-223
Identification Number:	10.1016/j.aca.2011.06.018
Status:	Peer Reviewed
Publication Status:	Published
Funder:	Czech Republic. Ministerstvo školství, mládeže a tělovýchovy [Ministry of Education, Youth, and Sport], European Regional Development Fund (ERDF), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
Grant number:	MSM6198959216 (MSM), CZ.1.05./2.1.00/01.0007 (ERDF), BB/F014651/1 (BBSRC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/38473