The Library

Prussian Blue acts as a mediator in a reagentless cytokinin biosensor

Tools

Kowalska, Marta, Tian, Faming, Šmehilová, Mária, Galuszka, Petr, Frébort, Ivo, Napier, Richard M. and Dale, Nicholas. (2011) Prussian Blue acts as a mediator in a reagentless cytokinin biosensor. Analytica Chimica Acta, Vol.701 (No.2). pp. 218-223. ISSN 0003-2670

Preview

PDF
WRAP_Kowalska_0380313-lf-101111-kowalska_et_al.pdf - Accepted Version - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (399Kb)

Official URL: http://dx.doi.org/10.1016/j.aca.2011.06.018

Abstract

An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzes an irreversible cleavage of N(6)-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare purified AtCKX2 as the basis of the cytokinin biosensor. Prussian Blue (PrB) was electrodeposited on Pt microelectrodes prior to deposition of the enzyme in a sol-gel matrix. The biosensor gave amperometric responses to several cytokinins. These responses depended on the presence of both the enzyme and the Prussian Blue. Thus Prussian Blue must act as an electron mediator between the FAD centre in CKX2 and the Pt surface.

[error in script] [error in script]

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Biosensors -- Design, Cytokinins, Prussian blue, Bioelectrochemistry
Journal or Publication Title:	Analytica Chimica Acta
Publisher:	Elsevier Science BV
ISSN:	0003-2670
Date:	9 September 2011
Volume:	Vol.701
Number:	No.2
Page Range:	pp. 218-223
Identification Number:	10.1016/j.aca.2011.06.018
Status:	Peer Reviewed
Publication Status:	Published
Funder:	Czech Republic. Ministerstvo školství, mládeže a tělovýchovy [Ministry of Education, Youth, and Sport], European Regional Development Fund (ERDF), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
Grant number:	MSM6198959216 (MSM), CZ.1.05./2.1.00/01.0007 (ERDF), BB/F014651/1 (BBSRC)
References:	[1] O. Hedden, Annu. Rev. Plant Physiol. Plant Mol. Biol. 44 (1993) 107–130. [2] G.C. Davis, M.B. Hein, B.C. Neely, C.R. Sharp, M.G. Carnes, Anal. Chem. 63 (1985) 639A–648A. [3] J. Olsson, K. Claeson, B. Karlberg, A.C. Nordström, J. Chromatogr. A 824 (1998) 231–239. [4] B. Sotta, G. Pillate, F. Pelese, I. Sabbagh, M. Bonnet, R. Maldiney, Plant Physiol. 84 (1987) 571–573. [5] H. Wabiko, M. Minemura, Plant Physiol. 112 (1996) 939–951. [6] L. River, A. Crozier, Principles and Practice of Hormone Analysis, Academic Press, London, 1987. [7] E.W. Weiler, J. Eberle, R. Mertens, R. Atzon, M. Feyerabend, P.S. Jordan, A. Arnscheidt, U. Wieczorek, Immunology in Plant Science, Cambridge Press, Cambridge, 1986. [8] E.M.S. MacDonail, R.O. Morris, Methods Enzymol. 110 (1985) 347–358. [9] L. Clark, C. Lyons, Ann. N. Y. Acad. Sci. 148 (1962) 133–153. [10] M. Pohanka, P. Skládal, J. Appl. Biomed. 6 (2008) 57–64. [11] E. Bakker, Anal. Chem. 76 (2004) 3285–3298. [12] A.A. Karyakin, E.E. Karyakin, L. Gorton, Anal. Chem. 72 (2000) 1720–1723. [13] Q. Deng, S.J. Dong, J. Electroanal. Chem. 377 (1994) 191–195. [14] L. Chen, W. Gorski, Anal. Chem. 73 (2001) 2862–2868. [15] X. Wei, J. Cruz, W. Gorski, Anal. Chem. 74 (2002) 5039–5046. [16] X. Wei, M. Zhang, W. Gorski, Anal. Chem. 75 (2003) 2060–2064. [17] I. Gill, A. Ballesteros, Trends Biotechnol. 18 (2000) 282–296. [18] R. Gupta, N.K. Chaudhury, Biosens. Bioelectron. 22 (2007) 2387–2399. [19] F. Tian, W. Wu, M. Broderick, V. Vamvakaki, N. Chaniotakis, N. Dale, Biosens. Bioelectron. 25 (2010) 2013–2408. [20] D.W. Mok, M.C. Mok, Annu. Rev. Plant Physiol. Plant Mol. Biol. 52 (2001) 89–118. [21] J. Li, L.-T. Xiao, G.-M. Zeng, G.-H. Huang, G.-L. Shen, R.-Q. Yu, Anal. Biochem. 321 (2003) 89–95. [22] B.G. Brownlee, R.H. Hall, C.D. Whitty, Can. J. Biochem. 53 (1975) 37–41. [23] H. Popelková, M.W. Fraaije, O. Novák, J. Frébortová, K.D. Bilyeu, I. Frébort, Biochem. J. 398 (2006) 113–124. [24] J. Frébortová, O. Novák, I. Frébort, R. Jorda, Plant J. 61 (2010) 467–481. [25] J. Frébortová, P. Galuszka, T. Werner, T. Schmülling, I. Frébort, Biol. Plant 51 (2007) 673–682. [26] T. Werner, V. Motyka, M. Strnad, T. Schmülling, Proc. Natl. Acad. Sci. USA 98 (2001) 10487–10492. [27] J.D. Bendtsen, H. Nielsen, G. von Heijne, S. Brunak, Mol. Biol. 340 (2004) 783–795. [28] M. Kowalska, P. Galuszka, J. Frébortová, M. ˇSebela, T. Béres, T. Hluska, M. ˇSmehilová, K.D. Bilyeu, I. Frébort, Phytochemistry 71 (2010) 1970–1978. [29] M.M. Bradford, Anal. Biochem. 72 (1976) 248–254. [30] J. Lin-Cereghino, J.M. Cregg, FEMS Microbiol. Rev. 24 (2000) 45–66. [31] G.P. Lin-Cereghino, J. Lin-Cereghino, Ch. Ilgen, J.M. Cregg, Curr. Opin. Biotechnol. 13 (2002) 329–332. [32] J. Lin-Cereghino, G.P. Lin-Cereghino, in: J.M. Cregg (Ed.), Pichia Protocols, Humana Press, Totowa, New Jersey, 2007, pp. 11–27. [33] X. Wang, H. Gu, F. Yin, Y. Tu, Biosens. Bioelectron. 24 (2009) 1527–1530. [34] F. Tian, A.V. Gourine, R.T.R. Huckstepp, N. Dale, Anal. Chim. Acta 645 (2009) 86–91. [35] M. Klotz, A. Ayral, C. Guizard, L. Cot, Bull. Korean Chem. Soc. 20 (1999) 879–884. [36] B. Yin, R. Yuan, Y. Chai, S. Chen, S. Cao, Y. Xu, P. Fu, Biotechnol. Lett. 30 (2008) 317–322. [37] J. Kulys, R. Stupak, Open Nanosci. J. 2 (2008) 34–38. [38] F. Ricci, F. Arduini, A. Amine, D. Moscone, G. Palleschi, J. Electroanal. Chem. (2004) 229–237. [39] F. Ricci, G. Palleschi, Biosens. Bioelectron. 21 (2005) 389–407. [40] F. Tian, E. Llaudet, N. Dale, Anal. Chem. 27 (2007) 6760–6766. [41] P. Galuszka, I. Frébort, M. ˇSebela, P. Sauer, S. Jacobsen, P. Peˇc, Eur. J. Biochem. 268 (2001) 450–461. [42] K.D. Bilyeu, J.L. Cole, J.G. Laskey, W.R. Riekhof, T.J. Esparza, M.D. Kramer, R.O. Morris, Plant Physiol. 125 (2001) 378–386. [43] J.G. Laskey, P. Patterson, K. Bilyeu, R.O. Morris, Plant Growth Regul. 40 (2003) 189–196. [44] J. Frébortová, M.W. Fraaije, P. Galuszka, M. ˇSebela, P. Peˇc, J. Hrbáˇc, K.D. Bilyeu, J.T. English, I. Frébort, Biochem. J. 380 (2004) 121–130. [45] J.C. Goodrick, M. Xu, R. Finnegan, S. Schilling, S. Schiavi, H. Hoppe, N.C. Wan, Biotechnol. Bioeng. 74 (2001) 492–497.
URI:	http://wrap.warwick.ac.uk/id/eprint/38473