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Sequence-specific predictive chromatography to assist mass spectrometric analysis of asparagine deamidation and aspartate isomerization in peptides
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Sargaeva, Nadezda P., Goloborodko, Anton A., O'Connor, Peter B., Moskovets, Eugene and Gorshkov, Mikhail V. (2011) Sequence-specific predictive chromatography to assist mass spectrometric analysis of asparagine deamidation and aspartate isomerization in peptides. Electrophoresis, Vol.32 (No.15). pp. 1962-1969. doi:10.1002/elps.201000507 ISSN 0173-0835.
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Official URL: http://dx.doi.org/10.1002/elps.201000507
Abstract
Deamidation of asparagine and spontaneous isomerization of aspartic acid in proteins and peptides occur frequently. These modifications result in a mixture of peptide variants containing all three residues in the sequences. Identification and isomer quantification for these systems are challenging tasks for tandem mass spectrometry commonly utilized in protein analysis. Chromatographic data provide a set of sequence-specific information complementary to mass spectrometry. In order to compare measured retention times (RTs) with those calculated from the sequences derived from protein databases, it is necessary to develop chromatographic models and tools allowing the prediction of RT and elution order for peptides with modified residues. In this work we extended recently introduced critical liquid chromatography of biomacromolecule model for prediction of RTs for peptides containing asparagines, aspartic acid, and isoaspartic acid residues.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
| Library of Congress Subject Headings (LCSH): | Chromatographic analysis, Biochemical markers, Isoaspartic acid -- Synthesis, Proteomics, Electrophoresis, Amino acids, Isomerization, Deamination, Peptides | ||||
| Journal or Publication Title: | Electrophoresis | ||||
| Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA | ||||
| ISSN: | 0173-0835 | ||||
| Official Date: | August 2011 | ||||
| Dates: |
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| Volume: | Vol.32 | ||||
| Number: | No.15 | ||||
| Number of Pages: | 8 | ||||
| Page Range: | pp. 1962-1969 | ||||
| DOI: | 10.1002/elps.201000507 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Warwick University Chemistry Department , Warwick Centre for Analytical Science (EPSRC) , National Institutes of Health (U.S.) (NIH), Rossiĭskiĭ fond fundamentalʹnykh issledovaniĭ [Russian Foundation of Basic Research] (RFBR) | ||||
| Grant number: | EP/F034210/1 (EPSRC), 09-04-00633 (RFSR), 08-04-01339 (RFBR) |
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