Sargaeva, Nadezda P., Goloborodko, Anton A., O'Connor, Peter B., Moskovets, Eugene and Gorshkov, Mikhail V.. (2011) Sequence-specific predictive chromatography to assist mass spectrometric analysis of asparagine deamidation and aspartate isomerization in peptides. Electrophoresis, Vol.32 (No.15). pp. 1962-1969. ISSN 0173-0835

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Abstract

Deamidation of asparagine and spontaneous isomerization of aspartic acid in proteins and peptides occur frequently. These modifications result in a mixture of peptide variants containing all three residues in the sequences. Identification and isomer quantification for these systems are challenging tasks for tandem mass spectrometry commonly utilized in protein analysis. Chromatographic data provide a set of sequence-specific information complementary to mass spectrometry. In order to compare measured retention times (RTs) with those calculated from the sequences derived from protein databases, it is necessary to develop chromatographic models and tools allowing the prediction of RT and elution order for peptides with modified residues. In this work we extended recently introduced critical liquid chromatography of biomacromolecule model for prediction of RTs for peptides containing asparagines, aspartic acid, and isoaspartic acid residues.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology Q Science > QP Physiology
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Chromatographic analysis, Biochemical markers, Isoaspartic acid -- Synthesis, Proteomics, Electrophoresis, Amino acids, Isomerization, Deamination, Peptides
Journal or Publication Title:	Electrophoresis
Publisher:	Wiley - V C H Verlag GmbH & Co. KGaA
ISSN:	0173-0835
Official Date:	August 2011
Volume:	Vol.32
Number:	No.15
Number of Pages:	8
Page Range:	pp. 1962-1969
Identification Number:	10.1002/elps.201000507
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Warwick University Chemistry Department , Warwick Centre for Analytical Science (EPSRC) , National Institutes of Health (U.S.) (NIH), Rossiĭskiĭ fond fundamentalʹnykh issledovaniĭ [Russian Foundation of Basic Research] (RFBR)
Grant number:	EP/F034210/1 (EPSRC), 09-04-00633 (RFSR), 08-04-01339 (RFBR)
URI:	http://wrap.warwick.ac.uk/id/eprint/38499

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