Domanov, Y. A., Aimon, Sophie, Toombes, G. E. S., Renner, Marianne, Quemeneur, F., Triller, Antoine, Turner, Matthew S. and Bassereau, P. (2011) Mobility in geometrically confined membranes. Proceedings of the National Academy of Sciences, Vol.108 (No.31). pp. 12605-12610. doi:10.1073/pnas.1102646108

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Abstract

Lipid and protein lateral mobility is essential for biological function. Our theoretical understanding of this mobility can be traced to the seminal work of Saffman and Delbruck, who predicted a logarithmic dependence of the protein diffusion coefficient (i) on the inverse of the size of the protein and (ii) on the "membrane size" for membranes of finite size [Saffman P, Delbruck M (1975) Proc Natl Acad Sci USA 72:3111-3113]. Although the experimental proof of the first prediction is a matter of debate, the second has not previously been thought to be experimentally accessible. Here, we construct just such a geometrically confined membrane by forming lipid bilayer nanotubes of controlled radii connected to giant liposomes. We followed the diffusion of individual molecules in the tubular membrane using single particle tracking of quantum dots coupled to lipids or voltage-gated potassium channels KvAP, while changing the membrane tube radius from approximately 250 to 10 nm. We found that both lipid and protein diffusion was slower in tubular membranes with smaller radii. The protein diffusion coefficient decreased as much as 5-fold compared to diffusion on the effectively flat membrane of the giant liposomes. Both lipid and protein diffusion data are consistent with the predictions of a hydrodynamic theory that extends the work of Saffman and Delbruck to cylindrical geometries. This study therefore provides strong experimental support for the ubiquitous Saffman-Delbruck theory and elucidates the role of membrane geometry and size in regulating lateral diffusion.

Item Type:	Journal Article
Subjects:	Q Science > QC Physics Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Science > Physics
Library of Congress Subject Headings (LCSH):	Membranes (Biology) -- Fluidity, Cell membranes, Bilayer lipid membranes, Diffusion, Membrane proteins, Brownian motion processes
Journal or Publication Title:	Proceedings of the National Academy of Sciences
Publisher:	National Academy of Sciences
ISSN:	0027-8424
Official Date:	August 2011
Dates:	Date Event August 2011 Published
Volume:	Vol.108
Number:	No.31
Page Range:	pp. 12605-12610
DOI:	10.1073/pnas.1102646108
Status:	Peer Reviewed
Publication Status:	Published
Funder:	France. Agence nationale de la recherche (ANR), Fondation Pierre-Gilles de Gennes (FPGG)
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