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Analysis of photosynthetic picoeukaryote community structure along an extended Ellett Line transect in the northern North Atlantic reveals a dominance of novel prymnesiophyte and prasinophyte phylotypes

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Kirkham, Amy R., Jardillier, Ludwig E., Holland, Ross, Zubkov, Mikhail V. and Scanlan, Dave J.. (2011) Analysis of photosynthetic picoeukaryote community structure along an extended Ellett Line transect in the northern North Atlantic reveals a dominance of novel prymnesiophyte and prasinophyte phylotypes. Deep Sea Research Part I: Oceanographic Research Papers, Vol.58 (No.7). pp. 733-744. ISSN 0967-0637

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Official URL: http://dx.doi.org/10.1016/j.dsr.2011.05.004

Abstract

Photosynthetic picoeukaryotes (PPEs) of a size <3 mu m can contribute significantly to primary production. Here, PPE community structure was analysed along an extended Ellett Line transect, an area in the North Atlantic well studied by physical oceanographers but largely neglected in the field of microalgal ecology. Distribution patterns of specific PPE classes were determined using dot-blot hybridization analysis, while the taxonomic composition of specific PPE classes was revealed by phylogenetic analysis of plastid 16S rRNA gene sequences. In addition, we performed fluorescent in situ hybridization (FISH) analysis of seawater samples collected along the transect to provide a PCR-independent survey of class level PPE distribution patterns. We found the PPE community was dominated by members of the Prymnesiophyceae, Prasinophyceae and Mamiellophyceae. Interestingly, phylogenetic analysis revealed several novel Prymnesiophyceae and Prasinophyceae phylotypes (with only 85-96% identity to neighbouring sequences) within lineages for which cultured counterparts are unknown

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Marine phytoplankton -- Research, Marine ecology -- North Atlantic Ocean, Photosynthesis -- Research, Eukaryotic cells -- Research, Algae -- Research, Polymerase chain reaction
Journal or Publication Title: Deep Sea Research Part I: Oceanographic Research Papers
Publisher: Pergamon
ISSN: 0967-0637
Date: July 2011
Volume: Vol.58
Number: No.7
Page Range: pp. 733-744
Identification Number: 10.1016/j.dsr.2011.05.004
Status: Peer Reviewed
Publication Status: Published
Funder: Natural Environment Research Council (Great Britain) (NERC)
Grant number: NE/C003160/1 (NERC)
URI: http://wrap.warwick.ac.uk/id/eprint/38583

Data sourced from Thomson Reuters' Web of Knowledge

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