Markovic, Danijela, Vatish, Manu, Gu, Mei, Slater, Donna, Newton, Rob, Lehnert, Hendrik and Grammatopoulos, Dimitris K.. (2007) The onset of labor alters corticotropin-releasing hormone type 1 receptor variant expression in human myometrium : putative role of interleukin-1ß. Endocrinology, Vol.148 (No.7). pp. 3205-3213. ISSN 0013-7227

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Abstract

CRH targets the human myometrium during pregnancy. The efficiency of CRH actions is determined by expression of functional receptors (CRH-R), which are dynamically regulated. Studies in myometrial tissue biopsies using quantitative RT-PCR demonstrated that the onset of labor, term or preterm, is associated with a significant 2- to 3-fold increase in CRH-R1 mRNA levels. Detailed analysis of myometrial CRH-R1 mRNA variants showed a decline of the pro-CRH-R1 mRNA encoding the CRH-R1ß variant during labor and increased mRNA levels of CRH-R1d mRNA. Studies in myometrial cells identified IL-1ß as an important regulator of myometrial CRH-R1 gene expression because prolonged treatment of myometrial cells with IL-1ß (1 ng/ml) for 18 h induced expression of CRH-R1 mRNA levels by 1.5- to 2-fold but significantly attenuated CRH-R1ß mRNA expression by 70%. In contrast, IL-1ß had no effect on CRH-R1d mRNA expression. Studies using specific inhibitors suggest that ERK1/2, p38 MAPK, and downstream nuclear translocation of nuclear factor-B mediate IL-1ß effects on myometrial CRH-R1 gene. However, the increased CRH-R1 mRNA expression was associated with a dampening of the receptor efficacy to activate the adenylyl cyclase/cAMP signaling cascade. Thus, our findings suggest that IL-1ß is an important regulator of CRH-R1 expression and functional activity, and this interaction might play a role in the transition of the uterus from quiescence to active contractions necessary for the onset of parturition.

Item Type:	Journal Article
Subjects:	R Medicine > RG Gynecology and obstetrics
Divisions:	Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Corticotropin releasing hormone, Myometrium, Labor (Obstetrics) -- Endocrine aspects, Interleukin-1
Journal or Publication Title:	Endocrinology
Publisher:	The Endocrine Society
ISSN:	0013-7227
Date:	July 2007
Volume:	Vol.148
Number:	No.7
Page Range:	pp. 3205-3213
Identification Number:	10.1210/en.2007-0095
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Wellcome Trust (London, England), Warwick Private Hospitals Charitable Trust
References:	1. Challis JRG, Matthews SG, Gibb W, Lye SJ 2000 Endocrine and paracrine regulation of birth at term and preterm. Endocr Rev 21:514–550 2. McLean M, Bisits A, Davies J, Woods R, Lowry P, Smith R 1995 A placental clock controlling the length of human pregnancy. Nat Med 1:460–463 3. Grammatopoulos D, Hillhouse EW 1999 Role of corticotropin-releasing hormone in the onset of labour. Lancet 353:1546–1549 4. Hillhouse EW, GrammatopoulosD 2001 Control of intracellular signalling by corticotropin-releasing hormone (CRH) in human myometrium. Front Horm Res 27:66–74 5. Hillhouse EW, Grammatopoulos D, Milton N, Quartero H 1993 The identification of a human myometrial corticotropin-releasing hormone receptor which increases in affinity during pregnancy. J Clin Endocrinol Metab 76: 736–741 6. Aggelidou E, Hillhouse EW, Grammatopoulos DK 2002 Up-regulation of nitric oxide synthase and modulation of the guanylate cyclase activity by corticotropin releasing hormone in cultured human pregnant myometrial cells. Proc Natl Acad Sci USA 99:3300–3305 7. Mignot TM, Paris B, Carbonne B, Vauge C, Ferre F, Vaiman D 2005 Corticotropin- releasing hormone effects on human pregnant vs. nonpregnant myometrium explants estimated from a mathematical model of uterine contraction. J Appl Physiol 99:1157–1163 8. Karteris E, Hillhouse EW, Grammatopoulos DK 2004 Expression of urocortin II (UCNII) and identification of distinct corticotropin-releasing hormone type-2 receptor (CRH-R2) signalling pathways regulating myosin light chain phosphorylation in pregnant myometrial cells. Endocrinology 145:890–900 9. Grammatopoulos D, Hillhouse EW 1999 Activation of protein kinase C by oxytocin inhibits the biological activity of the human myometrialCRHreceptor at term. Endocrinology 140:585–594 10. Markovic D, Papadopoulou N, Teli T, Levine MA, Hillhouse EW, Grammatopoulos DK 2006 Differential responses of CRH receptor type 1 variants to PKC phosphorylation. J Pharmacol Exp Ther 319:1–11 11. Hillhouse EW, Grammatopoulos DK 2006 The molecular mechanisms underlying the regulation of the biological activity of corticotropin-releasing hormone receptors: implications for physiology and pathophysiology. Endocr Rev 27:260–286 12. Grammatopoulos D, Dai Y, Chen J, Karteris E, Papadopoulou N, Easton AJ, Hillhouse EW 1998 Human CRH receptor: differences in subtype expression between pregnant and non-pregnant myometrium. J Clin Endocrinol Metab 83:2539–2544 13. Grammatopoulos D, Dai Y, Randeva HS, Karteris E, Levine MA, Easton AJ, Hillhouse EW 1999 A novel spliced variant of the type 1 corticotropin-releasing hormone (CRH) receptor with a deletion in the 7th transmembrane domain present in the human pregnant term myometrium and fetal membranes. Mol Endocrinol 13:2189–2202 14. Parham KL, Zervou S, Karteris E, Catalano RD, Old RW, Hillhouse EW 2004 Promoter analysis of human corticotropin-releasing factor (CRF) type 1 receptor and regulation by CRF and urocortin. Endocrinology 145:3971–3983 15. Lindstrom TM, Bennett PR 2005 The role of nuclear factor κB in human labour. Reproduction 130:569–581 16. Belt AR, Baldassare JJ, Molnar M, Romero R, Hertelendy F 1999 The nuclear transcription factor NF-κB mediates interleukin-1β-induced expression of cyclooxygenase- 2 in human myometrial cells. Am J Obstet Gynecol 181:359–366 17. Bartlett SR, Sawdy R, Mann GE 1999 Induction of cyclooxygenase-2 expression in human myometrial smooth muscle cells by interleukin-1β: involvement of p38 mitogen-activated protein kinase. J Physiol 520:399–406 18. Pande V, Ramos MJ 2005 NF-κB in human disease: current inhibitors and prospects for de novo structure based design of inhibitors. Curr Med Chem 12:357–374 19. Sooranna SR, Engineer N, Loudon JAZ, Terzidou V, Bennett PR, Johnson MR 2005 The mitogen-activated protein kinase dependent expression of prostaglandin H synthase-2 and interleukin-8 messenger ribonucleic acid by myometrial cells: the differential effect of stretch and interleukin-1β. J Clin Endocrinol Metab 90:3517–3527 20. Grammatopoulos D, Hillhouse EW 1999 Basal and interleukin-1β stimulated prostaglandin production from cultured human myometrial cells: differential regulation by corticotropin-releasing hormone. J Clin Endocrinol Metab 84: 2204–2211 21. Grammatopoulos D, Stirrat GM, Williams SA, Hillhouse, EW 1996 The biological activity of the corticotropin-releasing hormone receptor-adenylate cyclase complex in human myometrium is reduced at the end of pregnancy. J Clin Endocrinol Metab 81:745–751 22. Stevens MY, Challis JRG, Lye SJ 1998 Corticotropin-releasing hormone receptor subtype 1 is significantly up-regulated at the time of labour in the human myometrium. J Clin Endocrinol Metab 83:4107–4115 23. Pisarchik A, Slominski AT 2001 Alternative splicing of CRH-R1 receptors in human and mouse skin: identification of new variants and their differential expression. FASEB J 15:2754–2756 24. Grammatopoulos D, Thompson S, Hillhouse EW 1995 The human myometrium expresses multiple isoforms of the corticotropin releasing hormone receptor. J Clin Endocrinol Metab 80:2388–2393 25. Muhle RA, Pavlidis P, Grundy WN, Hirsch E 2001 A high-throughput study of gene expression in preterm labor with a subtractive microarray approach. Am J Obstet Gynecol 185:716–724 26. Viatour P, Merville MP, Bours V, Chariot A 2005 Phosphorylation of NF-κB and IκB proteins: implications in cancer and inflammation. Trends Biochem Sci 30:43–52 27. Soloff MS, Cook Jr DL, Jeng Y-J, Anderson GD 2004 In situ analysis of interleukin-1-induced transcription of COX-2 and IL-8 in cultured human myometrial cells. Endocrinology 145:1248–1254 28. Chapman NR, Europe-Finner GN, Robson SC 2004 Expression and deoxyribonucleic acid-binding activity of the nuclear factor κB family in the human myometrium during pregnancy and labor. J Clin Endocrinol Metab 89:5683– 5693. 29. Breuiller-Fouche M, Moriniere C, Dallot E, Oger S, Rebourcet R, Cabrol D, Leroy MJ 2005 Regulation of the endothelin/endothelin receptor system by interleukin-1β in human myometrial cells. Endocrinology 146:4878–4886 30. Oger S, Mehats C, Dallot E, Ferre F, Leroy MJ 2002 Interleukin-1β induces phosphodiesterase 4B2 expression in human myometrial cells through a prostaglandin E2- and cyclic adenosine 3',5'-monophosphate-dependent pathway. J Clin Endocrinol Metab 87:5524–5531 31. Chapman NR, Smyrnias I, Anumba DO, Europe-Finner GN, Robson SC 2005 Expression of the GTP-binding protein (Gαs) is repressed by the nuclear factor κB RelA subunit in human myometrium. Endocrinology 146:4994–5002
URI:	http://wrap.warwick.ac.uk/id/eprint/3865

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