Lopatář, Ján, Dale, Nicholas and Frenguelli, Bruno G.. (2011) Minor contribution of ATP P2 receptors to electrically-evoked electrographic seizure activity in hippocampal slices : evidence from purine biosensors and P2 receptor agonists and antagonists. Neuropharmacology, Vol.61 (No.1-2). pp. 25-34. ISSN 0028-3908

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Abstract

While the position of adenosine as an endogenous anticonvulsant is well established, it is unclear to what extent its precursor, ATP, contributes to seizure activity via P2 receptors. In this study we have addressed this issue through the use of ATP biosensors and agonists and antagonists of ATP P2 receptors to detect the release and role of ATP, respectively, during electrically-evoked electrographic seizure-like events (eSLEs) in rat hippocampal slices. The broad-spectrum P2 receptor antagonists RB-2 and PPADS (10 mu M) caused a small similar to 30% inhibition of eSLE duration, and a reduction in intensity. This inhibition of eSLEs was partially reproduced with the P2X(1,2/3,3) antagonist NF023 (10 mu M), but not the P2X(7) antagonist BBG (10 mu M). However, the P2X receptor agonist alpha,beta-meATP did not enhance eSLEs, but instead reduced their duration. Furthermore, we could discern no role for P2Y(1) receptors in electrically-evoked eSLEs: both the P2Y(1) antagonist MRS2179 (10 mu M) and the P2Y(1) receptor agonist 2-methylthioADP (10 mu M) were without effect on eSLEs. Consistent with a minor role for ATP P2 receptors on eSLEs we could detect no ATP release during eSLEs, although appreciable quantities of adenosine were detected, which had a pronounced inhibitory action on eSLEs via A(1) receptors. We conclude that the role of ATP P2 receptors in modulating electrographic seizure activity is limited, at least in models such as this one requiring electrical stimulation of afferent fibres. We further conclude that the presence and action of adenosine under these conditions may primarily reflect direct release of this purine.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Adenosine triphosphate, Biosensors, Epilepsy -- Treatment -- Research
Journal or Publication Title:	Neuropharmacology
Publisher:	Elsevier
ISSN:	0028-3908
Date:	July 2011
Volume:	Vol.61
Number:	No.1-2
Page Range:	pp. 25-34
Identification Number:	10.1016/j.neuropharm.2011.02.011
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Epilepsy Research UK
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URI:	http://wrap.warwick.ac.uk/id/eprint/38725

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