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Chromosomal macrodomains and associated proteins : implications for DNA organization and replication in gram negative bacteria

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Dame, Remus T. (Remus Thei), Kalmykowa, Olga J. and Grainger, David C.. (2011) Chromosomal macrodomains and associated proteins : implications for DNA organization and replication in gram negative bacteria. PLoS Genetics, Vol.7 (No.6). e1002123. ISSN 1553-7390

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Official URL: http://dx.doi.org/10.1371/journal.pgen.1002123

Abstract

The Escherichia coli chromosome is organized into four macrodomains, the function and organisation of which are poorly understood. In this review we focus on the MatP, SeqA, and SlmA proteins that have recently been identified as the first examples of factors with macrodomain-specific DNA-binding properties. In particular, we review the evidence that these factors contribute towards the control of chromosome replication and segregation by specifically targeting subregions of the genome and contributing towards their unique properties. Genome sequence analysis of multiple related bacteria, including pathogenic species, reveals that macrodomain-specific distribution of SeqA, SlmA, and MatP is conserved, suggesting common principles of chromosome organisation in these organisms. This discovery of proteins with macrodomain-specific binding properties hints that there are other proteins with similar specificity yet to be unveiled. We discuss the roles of the proteins identified to date as well as strategies that may be employed to discover new factors.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH426 Genetics
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Escherichia coli -- Genetics, Gram-negative bacteria -- Genetics, DNA replication
Journal or Publication Title:	PLoS Genetics
Publisher:	Public Library of Science
ISSN:	1553-7390
Date:	June 2011
Volume:	Vol.7
Number:	No.6
Page Range:	e1002123
Identification Number:	10.1371/journal.pgen.1002123
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Royal Society (Great Britain), Wellcome Trust (London, England), Nederlandse Organisatie voor Wetenschappelijk Onderzoek [Netherlands Organisation for Scientific Research] (NWO)
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URI:	http://wrap.warwick.ac.uk/id/eprint/38748