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Genetics of resistance to downy mildew in Brassica oleracea and breeding towards durable disease control for UK vegetable production

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Vicente, Joana G., Gunn, Nick, Bailey, Liz, Pink, David and Holub, E. B.. (2012) Genetics of resistance to downy mildew in Brassica oleracea and breeding towards durable disease control for UK vegetable production. Plant Pathology, Vol.61 (No.3). pp. 600-609. ISSN 0032-0862

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Official URL: http://dx.doi.org/10.1111/j.1365-3059.2011.02539.x

Abstract

Downy mildew resistance was previously identified from screening a Brassica oleracea collection against two standard UK isolates of Hyaloperonospora parasitica. Sources of resistance were chosen from this material and developed further in this study by generating doubled haploid (DH) and inbred lines. Seedlings from the new lines were tested for resistance to a larger collection of H. parasitica isolates collected in 2001-02 and 2007-08 from the main broccoli and cauliflower production regions of the UK. Three lines (derived from borecole or summer cabbage) were broadly resistant to the pathogen isolates. Three of the remaining lines exhibited strong isolate-specific resistance; several examples of weak or basal level of resistance to some isolates were observed. A new H. parasitica variant collected in 2008 was virulent in the broadly resistant lines, but was avirulent in a line with narrow specificity of resistance. The F2 and BC1 seedlings derived from outcrossing each of the three broadly resistant lines to susceptible broccoli and cauliflower lines segregated in a manner indicating that the resistance is controlled by a single dominant gene. No susceptibility was observed amongst F2 seedlings derived from intercrossing the three resistant lines, indicating that they all share the same or closely linked broad spectrum resistance gene(s). DH lines were produced from F1 plants and resistant plants were further back-crossed to produce broccoli and cauliflower-like lines that could be useful pre-breeding material. A combination of resistance from lines with broad and narrow specificity is recommended for controlling downy mildew in UK brassica production.

Item Type:

Journal Article

Subjects:

Q Science > QK Botany
S Agriculture > SB Plant culture

Divisions:

Faculty of Science > Life Sciences (2010- )
Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)

Library of Congress Subject Headings (LCSH):

Downy mildew diseases -- Genetic aspects, Downy mildew diseases -- Prevention, Brassica -- Disease and pest resistance -- Genetic aspects

Journal or Publication Title:

Plant Pathology

Publisher:

Wiley-Blackwell Publishing Ltd.

ISSN:

0032-0862

Official Date:

June 2012

Dates:

Date	Event
June 2012	Published

Volume:

Vol.61

Number:

No.3

Page Range:

pp. 600-609

Identification Number:

10.1111/j.1365-3059.2011.02539.x

Status:

Peer Reviewed

Publication Status:

Published

Access rights to Published version:

Restricted or Subscription Access

Funder:

Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA), European Union (EU)

Grant number:

HH0916SFV (DEFRA), HH3723SX (DEFRA), AIR3-CT920463 (EU)

References:

Bohuon EJR, Keith DJ, Parkin IAP, Sharpe AG, Lydiate DJ, 1996. Alignment of the conserved C genomes of Brassica oleracea and Brassica napus. Theoretical and Applied Genetics, 93, 833-839.
Borhan MH, Gunn N, Cooper A, Gulden S, Tor M, Rimmer SR, Holub EB, 2008. WRR4 encodes a TIR-NB-LRR protein that confers broad-spectrum white rust resistance in Arabidopsis thaliana to four physiological races of Albugo candida. Molecular Plant-Microbe Interactions, 21, 757-768.
Borhan MH, Holub EB, Kindrachuk C, Omidi M, Bozorgmanesh-Frad G, Rimmer SR, 2010. WRR4, a broad-spectrum TIR-NB-LRR gene from Arabidopsis thaliana that confers white rust resistance in transgenic oilseed brassica crops. Molecular Plant Pathology, 11, 283-291.
Brophy TF, Laing MD, 1992. Screening of fungicides for the control of downy mildew on container-grown cabbage seedlings. Crop Protection, 11, 160-164.
Carlsson M, Von Bothmer R, Merker A, 2004. Screening and evaluation of resistance to downy mildew (Peronospora parasitica) and clubroot (Plasmodiophora brassicae) in genetic resources of Brassica oleracea. Hereditas, 141, 293-300.
Channon AG, 1981. Downy mildew of Brassicas. In: Spencer DM, ed. The Downy Mildews. London: Academic Press, 321-339.
Coelho PS, Monteiro AA, 2003. Inheritance of downy mildew resistance in mature broccoli plants. Euphytica, 131, 65-69.
Crute IR, 1992. From breeding to cloning (and back again): a case study with lettuce downy mildew. Annual Review of Phytopathology, 30, 485-506.
Crute IR, Norwood JM, 1981. The identification and characteristics of field-resistance to lettuce downy mildew (Bremia lactucae Regel). Euphytica, 30, 707-717.
Ellis PR, Pink DAC, Phelps K, Jukes PL, Breeds SE, Pinnegar AE, 1998. Evaluation of a core collection of Brassica oleracea accessions for resistance to Brevicoryne brassicae, the cabbage aphid. Euphytica, 103, 149-160.
Farnham MW, Wang M, Thomas CE, 2002. A single dominant gene for downy mildew resistance in broccoli. Euphytica, 128, 405-407.
Hoser-Krauze J, Lakowska-Ryk E, Antosik J, 1995. The inheritance of resistance of some Brassica oleracea L. cultivars and lines to downy mildew Peronospora parasitica (Pers.) ex. Fr. Journal of Applied Genetics, 36, 27-33.
Hoser-Krauze J, Takowska-Ryk E, Antosik J, 1984. Resistance of cauliflower and broccoli (B. oleracea L. botrytis L.) seedlings to downy mildew - Peronospora parasitica. Cruciferae Newsletter, 9, 92-93.
Jensen BD, Hockenhull J, Munk L, 1999a. Seedling and adult plant resistance to downy mildew (Peronospora parasitica) in cauliflower (Brassica oleracea convar. botrytis var. botrytis). Plant Pathology, 48, 604-612.
Jensen BD, Vaerbak S, Munk L, Andersen SB, 1999b. Characterization and inheritance of partial resistance to downy mildew, Peronospora parasitica, in breeding material of broccoli, Brassica oleracea convar. botrytis var. italica. Plant Breeding, 118, 549-554.
Kou YJ, Wang SP, 2010. Broad-spectrum and durability: understanding of quantitative disease resistance. Current Opinion in Plant Biology, 13, 181-185.
Leckie D, Astley D, Crute IR, Ellis PR, Pink DAC, Boukema I, Monteiro AA, Dias S, 1996. The location and exploitation of genes for pest and disease resistance in European gene bank collections of horticultural brassicas. In: Dias JS, Crute I and Monteiro AA, eds. International Symposium on Brassicas - Ninth Crucifer Genetics Workshop. Leuven 1: International Society Horticultural Science, 95-101.
McIntosh RA, Brown GN, 1997. Anticipatory breeding for resistance to rust diseases in wheat. Annual Review of Phytopathology, 35, 311-326.
Mitchell-Olds T, James RV, Palmer MJ, Williams PH, 1995. Genetics of Brassica rapa (syn. campestris). 2. Multiple disease resistance to three fungal pathogens: Peronospora parasitica, Albugo candida and Leptosphaeria maculans. Heredity, 75, 362-369.
Moss NA, Crute IR, Lucas JA, Gordon PL, 1988. Requirements for analysis of host-species specificity in Peronospora parasitica (Downy mildew). Cruciferae Newsletter, 13, 114-116.
Natti JJ, Dickson MH, Atkins JD, 1967. Resistance of Brassica oleracea varieties to downy mildew. Phytopathology, 57, 144-147.
Norwood JM, Crute IR, Johnson AG, Gordon PL, 1983. A demonstration of the inheritance of field resistance to lettuce downy mildew (Bremia lactucae Regel) in progeny derived from cv. Grand Rapids. Euphytica, 32, 161-170.
Pink D, Bailey L, McClement S, Hand P, Mathas E, Buchanan-Wollaston V, Astley D, King G, Teakle G, 2008. Double haploids, markers and QTL analysis in vegetable brassicas. Euphytica, 164, 509-514.
Pink D, Puddephat I, 1999. Deployment of disease resistance genes by plant transformation - a 'mix and match' approach. Trends in Plant Science, 4, 71-75.
Pink DAC, 2002. Strategies using genes for non-durable disease resistance. Euphytica, 124, 227-236.
Schmit J, Taylor JD, Roberts SJ, 1993. Sources of resistance to pea bacterial blight (Pseudomonas syringae pv. pisi) in pea germplasm. In: 6th International Congress of Plant Pathology, Montreal, pp. 180.
Sharpe AG, Parkin IAP, Keith DJ, Lydiate DJ, 1995. Frequent nonreciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus). Genome, 38, 1112-1121.
Sherriff C, Lucas JA, 1990. The host range of isolates of downy mildew, Peronospora parasitica, from Brassica crop species. Plant Pathology, 39, 77-91.
Sivasithamparam K, Barbetti MJ, Li H, 2005. Recurring challenges from a necrotrophic fungal plant pathogen: a case study with Leptosphaeria maculans (causal agent of Blackleg disease in Brassicas) in Western Australia. Annals of Botany, 96, 363-377.
Stuthman DD, Leonard KJ, Miller-Garvin J, 2007. Breeding crops for durable resistance to disease. Advances in Agronomy, 95, 319-367.
Takahata Y, Keller WA, 1991. High frequency embryogenesis and plant regeneration in isolated microspore culture of Brassica oleracea L. Plant Science, 74, 235-242.
Taylor JD, Conway J, Roberts SJ, Astley D, Vicente JG, 2002. Sources and origin of resistance to Xanthomonas campestris pv. campestris in Brassica genomes. Phytopathology, 92, 105-111.
Taylor JD, Teverson DM, Davis JHC, 1996. Sources of resistance to Pseudomonas syringae pv. phaseolicola races in Phaseolus vulgaris. Plant Pathology, 45, 479-485.
Wang J, Lydiate DJ, Parkin IAP, Falentin C, Delourme R, Carion PWC, King GJ, 2011. Integration of linkage maps for the amphidiploid Brassica napus and comparative mapping with Arabidopsis and Brassica rapa. BMC Genomics, 12, 101.
Zhang NWW, Pelgrom K, Niks RE, Visser RGF, Jeuken MJW, 2009. Three combined quantitative trait loci from nonhost Lactuca saligna are sufficient to provide complete resistance of lettuce against Bremia lactucae. Molecular Plant-Microbe Interactions, 22, 1160-1168.