Aujla, Rajinder Kaur (1988) A study of rat olfactory mechanisms. PhD thesis, University of Warwick.

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Abstract

Membranes prepared from the olfactory mucosa of the rat
show a high level of adenylate cyclase activity, typically 500
pmol/min/mg protein. The activity increases in the presence
of odorants and is inhibited by calcium. Linkage of receptor
to cyclase seems to be mediated by a G-protein. The existence
of an odorant-stimulated adenylate cyclase in the rat is
discussed. The olfactory tissue is found to contain high
levels of intracellular cyclic AMP (ca. 40 pmoles/mg protein),
almost twice that found in the brain tissue. Odorants appear
to have no effect on these levels. The role of lipids in
olfaction is investigated. The olfactory epithelium is found
to be a rich source of phospholipids, comprising approximately
80% of the total lipids. The phospholipids identified are PC,
PE, PS, SM, PA, PI, PIP, PIP=, accounting for approximately
41,23,9,11,4,10,2, and 2%, respectively, of the total
phospholipids. 32P orthophosphate is incorporated into all
the phospholipids; PIP and PIP2 show the highest rate of
incorporation. Attempts are made to investigate the effect of
odorants on this rate of incorporation. The fatty acid
profile of the rat olfactory phospholipids is found not to be
unique to the olfactory tissue. Elaidic acid 81:1t [n-9],
however, seems to be present in higher concentrations than
observed in other tissues. The fatty acids identified are
14:0, 15:0, 16:0, 16:1[n-9], 17:0, 18:0, 18:1c[n-9], 18:1t [n-9], 18:2[n-6], 20:0, 20:3[n-6], 20:4[n-6], 20:5[n-3], 22:0,
22:4[n-6], 22:5[n-6], 22:5[n-3], 22:6[n-3], 24:0, and 24:1[n-9].

Item Type:	Thesis (PhD)
Subjects:	Q Science > QL Zoology
Library of Congress Subject Headings (LCSH):	Olfactory mucosa, Rats -- Physiology, Adenylate cyclase, Phospholipids
Official Date:	April 1988
Dates:	Date Event April 1988 Submitted
Institution:	University of Warwick
Theses Department:	Department of Chemistry
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Dodd, G. H.
Extent:	iii, 108 p.
Language:	eng

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