Li, Michael Kok Keng (2009) Quantitative analysis of chloroplast protein targeting. PhD thesis, University of Warwick.

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Abstract

This thesis presents the first use of the Partition of Unity Method in quantifying the spatio-temporal dynamics of a fluorescent protein targeted to the chloroplast twin-arginine translocation pathway. The fluorescence loss in photobleaching technique is applied in a modified fashion to the measurement of substrate mobilities in the chloroplast stroma. Our in vivo results address the two suggested protein targeting mechanisms of membrane-binding before lateral movement to the translocon and direct binding to the translocon. A high performance computing C/C++ implementation of the Partition of Unity Method is used to perform simulations of fluoresence loss in photobleaching and allow a compelling comparison to photobleaching data series. The implementation is both mesh-free and particle-less.

Item Type:	Thesis or Dissertation (PhD)
Subjects:	Q Science > QK Botany
Library of Congress Subject Headings (LCSH):	Partition of unity method, Chloroplasts, Fluorescent polymers, Plant proteins
Date:	September 2009
Institution:	University of Warwick
Theses Department:	Molecular Organisation and Assembly in Cells
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Robinson, Colin, 1958- ; Kirkilionis, Markus, 1962-
Sponsors:	Engineering and Physical Sciences Research Council (EPSRC) (EP/C512863/1), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) (BB/C00437X/1)
Extent:	xiii, 209 leaves : ill., charts
Language:	eng
URI:	http://wrap.warwick.ac.uk/id/eprint/3917

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