Quantitative analysis of chloroplast protein targeting
Li, Michael Kok Keng (2009) Quantitative analysis of chloroplast protein targeting. PhD thesis, University of Warwick.
WRAP_THESIS_Li_2009.pdf - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Official URL: http://webcat.warwick.ac.uk/record=b2341158~S9
This thesis presents the first use of the Partition of Unity Method in quantifying the spatio-temporal dynamics of a fluorescent protein targeted to the chloroplast twin-arginine translocation pathway. The fluorescence loss in photobleaching technique is applied in a modified fashion to the measurement of substrate mobilities in the chloroplast stroma. Our in vivo results address the two suggested protein targeting mechanisms of membrane-binding before lateral movement to the translocon and direct binding to the translocon. A high performance computing C/C++ implementation of the Partition of Unity Method is used to perform simulations of fluoresence loss in photobleaching and allow a compelling comparison to photobleaching data series. The implementation is both mesh-free and particle-less.
|Item Type:||Thesis or Dissertation (PhD)|
|Subjects:||Q Science > QK Botany|
|Library of Congress Subject Headings (LCSH):||Partition of unity method, Chloroplasts, Fluorescent polymers, Plant proteins|
|Institution:||University of Warwick|
|Theses Department:||Molecular Organisation and Assembly in Cells|
|Supervisor(s)/Advisor:||Robinson, Colin, 1958- ; Kirkilionis, Markus, 1962-|
|Sponsors:||Engineering and Physical Sciences Research Council (EPSRC) (EP/C512863/1), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) (BB/C00437X/1)|
|Extent:||xiii, 209 leaves : ill., charts|
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