Li, Michael Kok Keng (2009) Quantitative analysis of chloroplast protein targeting. PhD thesis, University of Warwick.

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Abstract

This thesis presents the first use of the Partition of Unity Method in quantifying
the spatio-temporal dynamics of a fluorescent protein targeted to
the chloroplast twin-arginine translocation pathway.
The fluorescence loss in photobleaching technique is applied in a modified
fashion to the measurement of substrate mobilities in the chloroplast
stroma. Our in vivo results address the two suggested protein targeting
mechanisms of membrane-binding before lateral movement to the translocon
and direct binding to the translocon.
A high performance computing C/C++ implementation of the Partition
of Unity Method is used to perform simulations of fluoresence loss
in photobleaching and allow a compelling comparison to photobleaching
data series. The implementation is both mesh-free and particle-less.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QK Botany
Library of Congress Subject Headings (LCSH):	Partition of unity method, Chloroplasts, Fluorescent polymers, Plant proteins
Official Date:	September 2009
Dates:	Date Event September 2009 Submitted
Institution:	University of Warwick
Theses Department:	Molecular Organisation and Assembly in Cells
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Robinson, Colin, 1958- ; Kirkilionis, Markus, 1962-
Sponsors:	Engineering and Physical Sciences Research Council (EPSRC) (EP/C512863/1), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) (BB/C00437X/1)
Extent:	xiii, 209 leaves : ill., charts
Language:	eng

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